萃取动力学光谱法测定癌组织中的微量元素

本文研究了在 p H9.2～ 9.5氨性缓冲溶液中 ,Cu( )催化 H2 O2 氧化溴酚蓝和丁基罗丹明 B等色染料离子对褪色的新指示反应 ,建立了较为广泛的快速终止催化反应的新方法。本方法测定铜 ,灵敏度为 0 .0 2μg·L- 1 ,线性范围为 0 .1～ 10 μg· L- 1。用于直肠癌组织中微量元素铜的测定 ,结果满意

Extraction-Catalytic Kinetic Spectrophotometric Determination of Trace Element in Tumor Tissue

In this paper an isochromatic dye ion-pairs extraction-catalytic spectrophotometric method for the determination of trace copper is studied. The method is based on the copper catalyzed oxidation of isochromatic dye ion-pairs formed by buthylrhodamine B and bromophenol blue by hydrogen peroxide in ammoniac medium. The reaction time, concentration of isochromatic dye ion-pairs in aqueous phase and degree of reaction are controlled by extraction equilibrium. The detection limit of this method is 0.02 microgram.L-1. The linear range is 0.1-10 micrograms.L-1. The method has been applied to the determination of trace copper in tumor tissue with satisfactory results.