The region Ser333-Arg356 of the alpha-chain of human C4b-binding protein is involved in the binding of complement C4b |
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Authors: | M Hessing D Kanters H Takeya C van 't Veer TM Hackeng S Iwanaga BN Bouma |
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Affiliation: | Department of Haematology, University Hospital Utrecht, The Netherlands. |
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Abstract: | Human C4b-binding protein (C4BP) functions as a cofactor to factor I in the degradation of C4b and accelerates the decay rate of the C4b2a complex. In this study we describe a monoclonal antibody directed against the alpha-chain of C4BP that inhibits the binding of C4b to C4BP. In order to identify the structural domain of the alpha-chain of C4BP that interacts with C4b, tryptic fragments of C4BP were generated. Amino acid sequence analysis of the fragments revealed that the residues Ser333-Arg356 of the alpha-chain of C4BP contain the epitope of this antibody, and as a consequence, that this part of the alpha-chain of C4BP is likely to be involved in the interaction with C4b. |
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