d-xylose transport by Candida succiphila and Kluyveromyces marxianus |
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Authors: | Boris U Stambuk Mary Ann Franden Arjun Singh Min Zhang |
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Affiliation: | (1) National Bioenergy Center, National Renewable Energy Laboratory, 1617 Cole Boulevard, 80401 Golden, CO;(2) Departamento de Bioquímica, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, SC, Brazil |
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Abstract: | The kinetics and regulation of d-xylose uptake were investigated in the efficient pentose fermentor Candida succiphila, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high-affinity (K
m
∼ 3.8 mM; V
max ∼ 15 nmol/mg·min]) and putative facilitated diffusion low-affinity (K
m
∼ 140 mM; V
max ∼ 130 nmol/mg·min]) transport activities were found in C. succiphila grown, respectively, on xylose or glucose. K. marxianus showed facilitated diffusion low-affinity (K
m
∼ 103 mM; V
max ∼ 190 nmol/mg·min]) transport activity when grown on xylose under microaerobic conditions, and both a low-affinity and an
active high-affinity (K
m
∼ 0.2 mM; V
max ∼ 10 nmol/mg·min]) transport activity when grown on xylose under fully aerobic conditions. |
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Keywords: | d-Xylose" target="_blank">d-Xylose transport kinetics fermentation Candida succiphila Kluyveromyces marxianus |
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