四逆汤煮散工艺研究

目的在四逆汤组成药材淡附片、干姜、甘草的最佳粒度条件下研究四逆汤的最佳煎煮工艺。方法取各药材的最佳粒度颗粒,采用HPLC法测定四逆汤中甘草酸铵的量,以紫外分光光度法测定四逆汤中总生物碱的量,采用单因素实验进行加水量、煎煮时间、煎煮次数等工艺考察,优选最佳煎煮工艺,再与其饮片进行对比,为临床合理使用煮散工艺提供依据。结果四逆汤的最佳煎煮工艺为浸泡20 min,加14倍量水,煎煮30 min,煎煮1次,且四逆汤煮散在所研究的每个时间点各成分的煎出率都比饮片大而其毒性不大于饮片,最佳条件下煮散各成分的煎出率也大于常规煎煮条件下的饮片。结论四逆汤煮散工艺具有节省药材、节约时间的优点,对解决目前中药材缺乏的问题有着不可忽视的作用。

Research on preparation technology for Sini Boiling Powder

Objective To optimize the extracting process for Sini Boiling Powder at the condition of optimum particle size. Methods The optimum size of particle was prepared, HPLC method was used for the determination of the content of ammonium glycyrrhetate and UV spectrophotometry was used for the determination of the content of total alkaloids. The decoction process was optimized by single factor test with boiling time, amount of water, and boiling times as factors. Then compared with traditional decoction, we can have a basis on clinic. Results The optimal conditions of decoction process were soaking 20 min,14-fold water, boiling 30 min, once. The results showed that both the yield of dry extract and the content of active ingredient at different time points from the boiling powder granule were greatly more then its traditional one during the whole decoction process, and it suggested that Sini Boiling Powder is more safe then Sini traditional decoction. Conclusion The advantage of high decoction rate and short preparing time on boiling powder can solve the problems that the traditional Chinese medicine is currently facing and should not be ignored.