蛛网膜下腔移植磁标骨髓间充质干细胞治疗脊髓损伤

背景：干细胞移植可以重建中枢神经系统的结构和功能,近年来引起了广泛的关注。目的：探讨超顺磁性氧化铁标记骨髓间充质干细胞对兔脊髓损伤神经功能恢复的作用。方法：采用密度梯度离心法和贴壁法体外分离培养兔骨髓间充质干细胞。制作兔脊髓损伤模型,并在蛛网膜下腔置管以备移植。将实验白兔随机分为3组：标记组移植超顺磁性氧化铁标记细胞;未标记组移植未标记细胞;对照组不移植细胞只注射PBS液。结果与结论：两移植组在细胞移植14d后运动功能BBB评分高于对照组,差异有显著性意义（P〈0.05）;但两组间差异无显著性意义（P〉0.05）。细胞移植后1,7,14,21,28,35d,脊髓损伤区域局部组织上出现大量含蓝色铁颗粒的细胞。经蛛网膜下腔移植标记骨髓间充质干细胞可定向迁移到脊髓损伤区域,并能促进脊髓损伤神经功能恢复。

Subarachnoid space transplantation of superparamagnetic iron oxide-labeled bone marrow mesenchymal stem cells for treatment of spinal cord injury

BACKGROUND： Transplantation of stem cells can rebuild the structure and function of injured central nervous system, and has attracted wide attention in recent years.
OBJECTIVE： To explore the effects of bone marrow mesenchymal stem cells labeled by superparamagnetic iron oxide on the recovery of neurological function in rabbits with spinal cord injury. METHODS： Bone marrow mesenchymal stem cells were isolated from rabbits, and cultivated in vitro using the density gradient centrifugation and the adherence in vitro separation. Passage 3 bone marrow mesenchymal stem cells were labeled with superparamagnetic iron oxide at 24 hours before use. Rabbit spinal cord injury models were made and the micro tubes were inserted into subarachnoid space. Rabbits were randomly divided into three groups.Rabbits from the group A were injected with superparamagnetic iron oxide labeled bone marrow mesenchymal stem cells via subarachnoid spaces. Rabbits from the group B received transplan tation of unlabeled bone marrow mesenchymal stem cells. Rabbits from the group C were injected with PBS and served as controls. At 1, 7, 14, 21, 28 and 35 days after cell transplantation, the recovery of neurological function was calculated by BBB scoring in all groups and the pathological tissue slices of spinal cord injury was examined. RESULTS AND CONCLUSION： The BBB scores in the groups A and V were significantly higher than those in the group C （P 〈 0.05）, but there was no statistical difference between groups A and B （P 〉 0.05）. At 1, 7, 14, 21, 28 and 35 days following cell transplantation, Prussian blue staining of tissue sections showed cells containing blue iron particles in the area of spinal cord injury. Bone marrow mesenchymal stem cells transplanted via subarachnoid space could migrate to the area of spinal cord injury, which thereby improved neurological function.