凋亡破骨细胞的拉伸应变

背景：力学应变在骨重建中起重要作用。然而，力学应变是否影响破骨细胞的凋亡仍不清楚。目的：观察力学应变对体外培养破骨细胞凋亡的影响。方法：对小鼠单核细胞RAW264．7采用巨噬细胞集落刺激因子和破骨细胞分化因子诱导，抗酒石酸酸性磷酸酶染色和骨吸收实验确定成功诱导出了破骨细胞。实验共分为3组，对诱导的破骨细胞分别施加0（对照组），2500和5000υε的基底拉伸应变3d，1h／次，1次／d。结果与结论：与对照组相比，生理强度载荷2500υε阻止了破骨细胞的凋亡和线粒体膜电位的下降。但是，病理强度载荷5000υε对破骨细胞的凋亡和线粒体膜电位没有影响。

Mechanical strain effects on osteoclast apoptosis in vitro

BACKGROUND： Mechanical strain plays an important role in bone remodeling. However, it remains unclear that whether mechanical strain has an influence on osteoclast apoptosis. OBJECTIVE： To investigate the effects of mechanical strain on osteoclast apoptosis in vitro. METHODS： RAW264.7 cells were treated with macrophage colony-stimulating factor and receptor activator of nuclear factor kappa-B ligand. Then, tartrate-resistant acid phosphatase staining and resorption assay were used to confirm that whether mature osteoclasts were induced successfully. Then the induced osteoclasts were subjected to 0, 2 500 and 5 000 υε mechanical stretch strain for 3 days, once for 1 hour per day. RESULTS AND CONCLUSION： Compared to the control group, 2 500 υε mechanical stretch strain inhibited osteoclast apoptosis and the collapse of mitochondrial membrane potential. But, the cells subjected to 5 000 υε mechanical stretch strain showed no significant differences compared to the control group.