| H5亚型禽流感病毒单抗-生物素捕获ELISA的建立 |
| |
| 引用本文： | 王传彬,田新生,曹振,遇秀玲,孙明,陈西钊,王宏伟,田克恭.H5亚型禽流感病毒单抗-生物素捕获ELISA的建立[J].实验动物与比较医学,2004,24(4):204-207. |
| |
| 作者姓名： | 王传彬 田新生 曹振 遇秀玲 孙明 陈西钊 王宏伟 田克恭 |
| |
| 作者单位： | 1. 复旦大学附属华山医院中西医结合肺与特应性疾病研究室,上海,200040
2. 上海市肺科医院,上海,200433 |
| |
| 基金项目： | 科技部高致病性禽流感防治攻关项目(02_02)，国家科技攻关计划项目(2004BA519A24)。 |
| |
| 摘 要： | 为制备过敏性哮喘模型，腹腔注射抗原(卵蛋白，OA)致敏豚鼠，2周后使豚鼠吸入雾化卵蛋白激发哮喘发作，以正常豚鼠为空白对照组，比较血、支气管肺泡灌洗液(BALF)中的嗜酸细胞及BALF细胞总数及气道反应性，并进行病理学检查以确定动物造模是否能模拟哮喘的病理状态。结果表明卵蛋白激发后，豚鼠的血、支气管肺泡灌洗液(BALF)中的嗜酸细胞总数较对照组增高，吸入组胺后气道内压较对照组明显增高，病理学结果显示为嗜酸性炎性浸润。
|
| 关 键 词： | 豚鼠 卵蛋白 BALF 对照组 吸入 嗜酸细胞 支气管肺泡灌洗液 动物 嗜酸性 模型建立 |
| 文章编号： | 1004-8448(2004)04-0204-03 |
| 修稿时间： | 2004/10/9 0:00:00 |
Establishment of H5 Avian Influenza Virus Capture ELISA Employing Biotin Labeled Monoclonal Antibody |
| |
| WANG Chuan_bin,TIAN Xin_sheng,CAO Zheng,YU Xiu_ling,SUN Ming,CHEN Xi_zhao,WANG Hong_wei,TIAN Ke_gong.Establishment of H5 Avian Influenza Virus Capture ELISA Employing Biotin Labeled Monoclonal Antibody[J].Laboratory Animal and Comparative Medicine,2004,24(4):204-207. |
| |
| Authors: | WANG Chuan_bin TIAN Xin_sheng CAO Zheng YU Xiu_ling SUN Ming CHEN Xi_zhao WANG Hong_wei TIAN Ke_gong |
| |
| Abstract: | To establish the allergic asthma model, guinea pigs were intraperitoneally injected with ovabulmin, and challenged by inhaling ovabulmin with ultrasonically nebulised inhaler two weeks later. The total number of cell and eosinphil count in brochial alveolar lavage fluid (BALF), eosinphil count in plasma, airway resistance ,pathology were detected. The results showed that eosinphil count in plasma and BALF of the model animal were significantly increased after the challenge compared with the control, intrapul monary pressure were increased after inhaling histamine, and eosinphil inflammion were found in pathological exanination. |
| |
| Keywords: | Guinea pig Allergic asthma Model |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《实验动物与比较医学》浏览原始摘要信息 |
|
点击此处可从《实验动物与比较医学》下载全文 |
