| 源于皱纹盘鲍裙边胶原蛋白ACE抑制肽的制备 |
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| 引用本文： | 刘维维,肖盼盼,陈玉磊,张凌晶,翁凌,刘光明,曹敏杰.源于皱纹盘鲍裙边胶原蛋白ACE抑制肽的制备[J].食品科学,2023,44(6):158-164. |
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| 作者姓名： | 刘维维 肖盼盼 陈玉磊 张凌晶 翁凌 刘光明 曹敏杰 |
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| 作者单位： | （集美大学海洋食品与生物工程学院，福建 厦门 361021） |
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| 基金项目： | “十三五”国家重点研发计划重点专项（2018YFD0901004）；国家自然科学基金面上项目（31772049） |
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| 摘 要： | 为了提高皱纹盘鲍(Haliotis discus hannai)裙边的利用率，以其为原料纯化胶原蛋白，并通过胰蛋白酶和胃蛋白酶共酶解制备血管紧张素转换酶(angiotensin I-converting enzyme,ACE)抑制肽。酶解液经超滤、SuperdexTM peptide10/300GL凝胶柱和高效液相色谱分离纯化，获得了3条来源于皱纹盘鲍胶原蛋白的肽段SGEVGQ、QRGPAGAQGPQ和GPPGPAGAR。其中结合能力最强的肽为GPPGPAGAR，对ACE的IC50值为177.1μmol/L，分子对接结果显示其主要作用于ACE的S1活性口袋，抑制模式与赖诺普利类似，并且经模拟胃肠液消化后仍能发挥较强的ACE抑制作用。本研究通过酶解皱纹盘鲍裙边胶原蛋白制备ACE抑制肽，为鲍鱼裙边的精深加工和ACE抑制肽的开发提供了参考。
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| 关 键 词： | 鲍鱼裙边 胶原蛋白 血管紧张素转换酶抑制肽 分子对接 |
Preparation of Angiotensin I-Converting Enzyme Inhibitory Peptides from Haliotis discus hannai Mantle Collagen |
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| LIU Weiwei,XIAO Panpan,CHEN Yulei,ZHANG Lingjing,WENG Ling,LIU Guangming,CAO Minjie.Preparation of Angiotensin I-Converting Enzyme Inhibitory Peptides from Haliotis discus hannai Mantle Collagen[J].Food Science,2023,44(6):158-164. |
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| Authors: | LIU Weiwei XIAO Panpan CHEN Yulei ZHANG Lingjing WENG Ling LIU Guangming CAO Minjie |
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| Affiliation: | (College of Marine Food and Biological Engineering, Jimei University, Xiamen 361021, China) |
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| Abstract: | In order to improve the utilization of Haliotis discus hannai mantle, collagen purified from it was hydrolyzed by trypsin and pepsin to prepare angiotensin I-converting enzyme (ACE) inhibitory peptides. The hydrolysate was purified by sequential ultrafiltration, SuperdexTM peptide 10/300 GL gel filtration chromatography and high performance liquid chromatography (HPLC). Three ACE inhibitory peptides, SGEVGQ, QRGPAGAQGPQ and GPPGPAGAR, were obtained . Among them, GPPGPAGAR had the highest ACE inhibitory activity with a half maximal inhibitory concentration (IC50) of 177.1 μmol/L. Molecular docking results showed that GPPGPAGAR mainly acted on the S1 active pocket of ACE and the inhibitory mode was similar to that of lisinopril. GPPGPAGAR still showed high ACE inhibitory activity after simulated gastrointestinal digestion. This study will provide a reference for deep processing of abalone mantle and the development of ACE inhibitory peptides. |
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| Keywords: | abalone mantle collagen angiotensin I-converting enzyme inhibitory peptide molecular docking |
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