果蝇新品系开发与种质资源保存

摘要: 黑腹果蝇( Drosophila melanogaster) 是当今生物医学研究领域最重要的模式生物之一。良好的饲养、高效的转基因构建和规范化的品系保存是顺利开展果蝇实验的三大基石。清华大学果蝇中心作为国内最大的果蝇资源平台，积累了丰富的果蝇操作和维护经验。本文综合介绍了清华大学果蝇中心在日常饲养、品系开发和种质保存等方面建立的标准化操作体系，旨在为广大科研同仁提供经验参考，加快我国在以果蝇为模式生物研究生物医学重大问题等领域的研究进程。

The Standard of Drosophila Strain Development and Preservation

Abstract: Drosophila melanogaster is one of the most important model organisms in biological and medical research．It is understood that the three pillars of successful Drosophila experiments are: proper culture condition，efficient transgenic technology，and standardized strain preservation． As the biggest fly resource platform in China，Tsinghua Fly Center ( THFC) is experienced in fly genetics and fly preservation． This article summarizes the standard methods of fly culture，new strain production and strain preservation developed by THFC，aiming to provide information for the Chinese Drosophila research community，and to expedite important biological and medical researches that use Drosophila as model organism．