上流锥形亲和层析床的流动特性研究

为了分析流体在上流锥形亲和层析床中的复杂流动情况,运用二相流理论建立了上流锥形床中液固二相流模型,对上流锥形床中的流动状况进行了数值模拟。运用FLUENT计算软件求解模型,得到了上流锥形床中直观的流场分布。分析了入口流速、溶液质量浓度及锥角等参数变化对流场的影响。以吸附剂的利用率和蛋白质的回收率为衡量标准,研究了流体流动状态改变对吸附的影响。运用计算流体力学方法分析亲和层析过程,为新型亲和层析设备的开发提供了崭新的思路。

Flowing characteristics of affinity adsorption in tapered bed with upflow method

In order to recognize the flow characteristics in a tapered affinity adsorption bed, FLUENT software was used in numerical simulation for the liquid-solid two phases flow. A two-fluid model of the liquid-solid in the tapered bed was established based on the theory of two-phase flow. The contours of inner velocity were obtained from this model. The effects of inlet velocity, solution concentration and angle were analyzed. The efficiencies were evaluated in terms of both solute recovery and the adsorbent utilization. The results showed that due to the existing of friction force between the wall and fluid, velocity near the wall is lower than that in center. Furthermore, the smaller the angle, the higher the expanded height; and the smaller the angle, the easier the overflow of particles. When operation is at the same angle tapered bed, expanded height will be higher along with increasing of inlet velocity and amount of particles in bed. Variation of protein concentration and adsorption reaction affected the fluid velocity a little.