贝那普利对肝纤维化大鼠胰岛素样生长因子-I受体的影响

目的研究血管紧张素转换酶抑制剂贝那普利对大鼠肝纤维化模型的疗效，以及对其肝组织胰岛素样生长因子-1受体（IGF—IR）的影响。方法取Wistar雄性大鼠42只随机分为3组，正常对照组12只，模型组15只，贝那普利治疗组15只。制备四氯化碳诱导的大鼠肝纤维化模型，同时应用贝那普利灌胃，共8周。对肝组织进行苏木精伊红染色及马松三色染色，观察各组肝纤维化的程度，并测定血清丙氨酸氨基转移酶（ALT）水平，采用SABC免疫组织化学方法检测大鼠肝组织中IGF—IR的表达水平。结果贝那普利治疗组的肝纤维化程度明显较同期模型组轻（P〈0．05）；与对照组相比，模型组体重降低（P〈0．05）及血清ALT升高（P〈0．05），IGF-IR在肝组织中表达明显增多（P〈0．05），贝那普利治疗组IGF—IR表达减少（P〈0．05）。结论贝那普利可改善肝纤维化程度，可能与肝组织IGF—IR的表达减少有关。

Effect of benazepril on insulin-like growth factor- I receptor of hepatic fibrosis in rats

Objective To explore the curative effects of angiotensin converting enzyme inhibitors benazepril in hepatic fibrosis induced by chronic carbon tetracbloricle （CC14） in rats and to observe the influence of benazepril on insulin-like growth factor- receptor（IGF i R）. Methods A total of 42 wistar male rats were randomly divided into three groups： the control group with 12 rats, the model group with 15 rats, and the benazepril treated group 15 rats. Except rats in control group, all were given subcutaneous injections of 40% CC14, and from the first day of the intraperitoneal injection, rats in treatment group were given benazepril for 8 weeks by gastric gavage, laver tissue and blood samples of all rats were examined at the end of 8 weeks. Blood sample were taken for detecting alanine aminotransferase （ALT） ,histopathological study of liver tissue was done with hematoxylin eosin （HE） and Masson trichrome staining. IGF- I R were evaluated by immunohistochemistry. Results Compared with the same period rats in model group, the liver fibrosis degree of the rats in treatment group was significantly reduced. The weight of the model group was decreased（P〈0.05）. Benazepril reduced AI.T（P〈0.05）. The expression of IGF- IR in liver tissue increased with the development of hepatic fibrosis, and benazepril decreased the expression of IGF- I R in hepatic fibrosis in rats（P〈0.05）. Conclusions Benazepril can delay the degree of hepatic fibrosis. It＇s probably relative to decrease the expression of IGF- I R.