一种改良小鼠脓毒症模型的建立

目的 建立一种模拟外科临床实际,早期致死率低,用于脓毒症综合治疗研究的小鼠模型.方法 小鼠随机分为3组,即对照组、盲肠结扎穿孔(CLP)组和改良组.对照组行假手术;CLP组行盲肠结扎穿孔术;改良组通过CLP后8 h行盲肠切除和腹腔生理盐水冲洗建立脓毒症模型.观察各组动物出现死亡时间及24、48、72 h累计死亡率;术后4、12、24 h分别处死动物,检测外周血白细胞计数(VC-BC),血清内毒素、肿瘤坏死因子(TNF)水平及肺、肝病理.结果 改良组动物出现死亡时间较CLP组明显延迟,术后48、72 h累计死亡率虽明显低于CLP组(P＜0.05),但仍分别高达53.3%、80%;术后各时间点WBC较CLP组与假手术组明显升高(P＜0.05);血清内毒素和TNF-α水平明显高于假手术组,但低于CLP组;光镜下见肺泡间隔增宽,间质充血、水肿、大量炎性细胞浸润,肝细胞水肿伴散在点状坏死,肝窦扩张伴间质大量炎性细胞浸润.结论 该脓毒症改良模型具有良好的外科临床相关性,早期致死率低,可重复性好.

Establishment of a moditled laboratory sepsis model in mice

Objective To establish a modified laboratory sepsis model in mice.Methods Male mice were randomly divided into 3 groups.Shamed operation was performed in control group,and cecal ligation and punction were performed in CLP group.In modified group,cecal ligation and punction were performed and 8 h later,cecum was excised and abdominal cavity was lavaged.Animal mortality was observed at 24,48 and 72 h after operation.White blood cell count(WBC)in peripheral blood,blood-serum levels of endotoxin and TNF-α were assayed,and lung and liver morphology was observed.Results Animal mortality at 48 and 72 h after operation in modified group was 53.5% and 80% respectively,which was significantly lower than in CLP group(P＜0.05).WBC in modified group was significantly more than in CLP group(P＜0.05).Blood-serum levels of endotoxin and TNF-α in modified group were markedly increased after operation,but significantly lower than in CLP group(P＜0.05).Meanwhile,enlarged alveolar septum,dilated hepatic sinusoid,hepatocyte necrosis and infiltration of inflammatory cells were observed in modified group.Conclusion The modified model has low mortality rate in the early stage and was more clinically relevant.