| 小鼠胚胎干细胞培养条件的探索 |
| |
| 引用本文： | 黄璟,何志旭.小鼠胚胎干细胞培养条件的探索[J].贵阳医学院学报,2006,31(2):109-112. |
| |
| 作者姓名： | 黄璟 何志旭 |
| |
| 作者单位： | 贵阳医学院附院,儿科,贵州,贵阳,550004 |
| |
| 摘 要： | 目的: 探讨小鼠胚胎干细胞(ES细胞)的培养特点及生物学特性,为ES细胞的应用研究奠定基础.方法: 小鼠胚胎成纤维细胞原代培养,3～5代的细胞作为饲养层细胞培养ES细胞.另在无饲养层细胞、明胶包被培养皿、培养液中含白血病抑制因子条件下培养小鼠ES细胞,观察2种情况下ES细胞的生长情况.结果: 小鼠胚胎成纤维细胞呈放射状或旋涡状走行,ES细胞在2种条件下均能呈克隆状生长,且保持未分化状态,碱性磷酸酶(AKP)染色呈阳性.结论: 2种条件下ES细胞均能良好生长.
|
| 关 键 词： | 干细胞 胚胎 细胞 培养的 小鼠 近交BALB C 胚胎干细胞 |
| 文章编号： | 1000-2707(2006)02-0109-04 |
| 收稿时间： | 2005-08-04 |
| 修稿时间： | 2006-01-13 |
Culture and Biological Characteristics of Mouse Embryonic Stem Cell |
| |
| HUANG Jing,HE Zhi-xu.Culture and Biological Characteristics of Mouse Embryonic Stem Cell[J].Journal of Guiyang Medical College,2006,31(2):109-112. |
| |
| Authors: | HUANG Jing HE Zhi-xu |
| |
| Affiliation: | Department of Pediatrics, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004. China |
| |
| Abstract: | Objective: To investigate the culture and biological characteristics of mouse embryonic stem(ES) cell as a foundational work for its application.Methods: Mouse ES cells were observed in two culture systems: 1) on feeder cells of the primary mouse embryo fibroblasts and 2) in leukemia with inhibititory factor but without feeder cells.Results: The primary mouse embryo fibroblast growth trended radially or circinately.The ES cell clones under the two conditions appeared typical characteristics and maintained un-differentiation state.Conclusion: The ES cells can grow well under those conditions. |
| |
| Keywords: | stem cells embryo cells cultured mice inbred BALB C embryonic stem cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
