| 高效液相色谱法测定辣椒中的辣椒碱和二氢辣椒碱 |
| |
| 引用本文： | 谷岩,刘广福,黄旭,陈晓迪,祝旭,白树田.高效液相色谱法测定辣椒中的辣椒碱和二氢辣椒碱[J].化学分析计量,2011,20(6):74-76. |
| |
| 作者姓名： | 谷岩 刘广福 黄旭 陈晓迪 祝旭 白树田 |
| |
| 作者单位： | 1. 辽宁省产品质量监督检验院,沈阳,110004
2. 辽宁省北票市质量技术监督局,辽宁北票,122000 |
| |
| 摘 要： | 建立了高效液相色谱法(HPLC)测定辣椒中辣椒碱、二氢辣椒碱的检测方法.实验考察了仪器条件、不同流动相体系、流动相配比、柱温、流速等因素对分离的影响.确定了最佳色谱条件为ZORBAX SB-C18色谱柱(250mm×4.6 mm,5μm)；二极管阵列检测器,辣椒碱、二氢辣椒碱的最佳检测波长为280 nm；色谱柱温度为2...
|
| 关 键 词： | 高效液相色谱 辣椒碱 二氢辣椒碱 |
DETERMINATION OF CAPSAICIN AND DIHYDROCAPSAICIN IN PEPPER BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
| |
| Gu Yan,Liu Guangfu,Huang Xu,Chert Xiaodi,Zhu Xu Liaoning Province Product Quality Supervision and Inspection Center,Shenyang,China Bai Shutian.DETERMINATION OF CAPSAICIN AND DIHYDROCAPSAICIN IN PEPPER BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J].Chemical Analysis And Meterage,2011,20(6):74-76. |
| |
| Authors: | Gu Yan Liu Guangfu Huang Xu Chert Xiaodi Zhu Xu Liaoning Province Product Quality Supervision and Inspection Center Shenyang China Bai Shutian |
| |
| Affiliation: | Gu Yan, Liu Guangfu, Huang Xu, Chert Xiaodi, Zhu Xu (Liaoning Province Product Quality Supervision and Inspection Center, Shenyang 110004, China) Bai Shutian (Liaoning Province Beipiao City Quality and Technical Supervision Bureau, Beipiao 122000, China) |
| |
| Abstract: | A method to determine capsaicin and dihydrocapsaicin in pepper products simultaneously by high performance liquid chromatography （HPLC） was developed. The effects of mobile phase composition, proportion, column temperature, velocity of flow and injection volume on separation were studied. The best chromatographic conditions were determined： a ZORBAX SB C18 column （250 mm × 4.6 mm, 5 μ m） ; a DAD detector ; the optimal detection wavelengths of capsaicin and dihydrocapsaicin were 280 nm ; the column temperature was 20℃ . The mobile phase was water-methanol（Volume ratio was 35 ： 65） with flow rate of 1.0 mL / min ; the injection volume was 5μL. Under the optimal conditions, calibration curves of each substitutes were established, and the related coefficients were 0.999 9. The recoveries of capsaicin and dihydrocapsaicin in samples were 94.1%-96.8%, 92.6%-- 95.0% respectively. The RSD was less than 2%（n=11）. The method has achieved the simultaneous determination on capsaicin and dihydrocapsaicin in 25 minutes. |
| |
| Keywords: | HPLC capsaicin dihydrocapsaicin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
