| COC1/DDP亚细胞中波形纤维蛋白的肽质谱指纹分析 |
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| 引用本文： | 吕磊,刘志强,刘淑莹,刘丽. COC1/DDP亚细胞中波形纤维蛋白的肽质谱指纹分析[J]. 宝鸡文理学院学报(自然科学版), 2006, 26(4): 279-281,324 |
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| 作者姓名： | 吕磊 刘志强 刘淑莹 刘丽 |
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| 作者单位： | 延安大学化学与化工学院,陕西,延安,716000;中国科学院,长春应用化学研究所,吉林,长春,130022;中国科学院,长春应用化学研究所,吉林,长春,130022;陕西延长石汕集团公司,延安炼油厂,陕西,延安,716000 |
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| 摘 要： | 目的寻找与鉴定卵巢癌耐药株中的差异表达蛋白质。方法应用高分辨二维凝胶电泳分离卵巢癌细胞中差异表达的波形纤维蛋白,并通过基质辅助激光解吸电离飞行时间质谱对其进行分析与鉴定。结果获得了分辨率和重复性均较佳的凝胶电泳图谱,图像分析显示波形纤维蛋白表达明显下调。结论与其他方法相比,应用凝胶电泳和生物质谱联用技术分析与鉴定未知蛋白具有简单快捷的特点。
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| 关 键 词： | 卵巢癌 波形纤维蛋白 二维凝胶电泳 基质辅助激光解吸电离飞行时间质谱 |
| 文章编号： | 1007-1261（2006）04-0279-03 |
| 收稿时间： | 2006-07-28 |
| 修稿时间： | 2006-07-282006-08-19 |
Identification of vimentin in subline COC1/DDP by MALDI-TOF mass peptide map analysis |
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| LLei,LIU Zhi-qing,LIU Shu-ying,LIU Li. Identification of vimentin in subline COC1/DDP by MALDI-TOF mass peptide map analysis[J]. Journal of Baoji College of Arts and Science(Natural Science Edition), 2006, 26(4): 279-281,324 |
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| Authors: | LLei LIU Zhi-qing LIU Shu-ying LIU Li |
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| Affiliation: | L(U)Lei,LIU Zhi-qing,LIU Shu-ying,LIU Li |
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| Abstract: | Aim To search and identify the different expressed protein of subline COC_1/DDP. Methods An obviously different expressed protein spot was separated by two dimensional electrophoresis, then analyzed and identified as vimentin using MALDI-TOF mass spectrometry. Results The good 2-DE images including resolution and reproducibility was obtained. The different protein expression analysis indicates that vimentin expression is obviously upregulated. Conclusion The results indicate that the method has more advantage for identification unknown protein than the others by using 2-DE gel electrophoresis tenique combined with bio-mass spectrometry. |
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| Keywords: | ovarian adenocarcinoma vimentin 2-DE gel MALDI-TOF-MS |
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