| 穿心莲二萜类化合物对B16细胞致敏小鼠CTL杀伤活性的影响 |
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| 引用本文： | 李满妹,徐炎,程燕,张坤,吕艳青,姚新生,栗原博.穿心莲二萜类化合物对B16细胞致敏小鼠CTL杀伤活性的影响[J].中国药理学通报,2007,23(5):688-692. |
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| 作者姓名： | 李满妹 徐炎 程燕 张坤 吕艳青 姚新生 栗原博 |
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| 作者单位： | 暨南大学中药及天然药物研究所,广东,广州,510632 |
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| 基金项目： | 广州市中药现代化研究重大科技专项资助项目;暨南大学人才引进资助项目 |
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| 摘 要： | 目的研究穿心莲二萜类化合物(DAP)对B16细胞致敏小鼠脾脏CTL杀伤活性的影响。方法将♂BALB/c小鼠分为对照组、致敏组、50mg·kg-1及150mg·kg-1DAP给药组,制备小鼠脾脏淋巴细胞悬液,流式细胞术分析T淋巴细胞亚群,乳酸脱氢酶(LDH)法和DiO/PI双染色法分别测定CTL杀伤活性。结果50mg·kg-1及150mg·kg-1DAP口服给药可以增加B16细胞致敏小鼠脾脏淋巴细胞中T细胞及CD8+T细胞比例,降低T细胞中CD4+/CD8+比值并增强CTL对B16细胞的杀伤活性。结论DAP可增加B16细胞致敏小鼠脾脏T细胞比例及提高CTL杀伤活性。
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| 关 键 词： | 穿心莲 二萜类化合物 B16细胞 CTL 流式细胞术 |
| 文章编号： | 1001-1978（2007）05-0688-05 |
| 修稿时间： | 2006-11-21 |
Effect of diterpenoids from Andrographis paniculata (Burm. F. ) nees on CTL activity in mice sensibilized by B16 cells |
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| LI Man-mei,XU Yan,CHENG Yan,ZHANG Kun,L Yan-qing,YAO Xin-sheng,Hiroshi kurihara.Effect of diterpenoids from Andrographis paniculata (Burm. F. ) nees on CTL activity in mice sensibilized by B16 cells[J].Chinese Pharmacological Bulletin,2007,23(5):688-692. |
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| Authors: | LI Man-mei XU Yan CHENG Yan ZHANG Kun L Yan-qing YAO Xin-sheng Hiroshi kurihara |
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| Affiliation: | 1. Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China |
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| Abstract: | Aim To study the effect of diterpenoids of Andrographis paniculata (DAP) on CTL activity in mice sensibilized by B16 cells. Methods The male BALB/c mice were randomly divided into untreated control group, sensibilized control group, 50 mg·kg-1 and 150 mg·kg-1 DAP group. The spleen lymphocyte suspensions of each group were prepared. Parameters of spleen T cells subset were measured by Flow cytometry and CTL activity was detected by LDH assay and DiO, PI dying analysis, respectively. Results 50 mg·kg-1 and 150 mg·kg-1 DAP could increase the percent of CD8+ T cells, decrease CD4+/CD8+ of T cells and moreover enhance the cytotoxic activity of CTL. Conclusion DAP can enhance CTL activity in mice sensibilized by B16 cells. |
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| Keywords: | CTL |
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