可高效降解甜高粱秸秆产糖的纤维素酶研究

为评价实验室自制的纤维素酶对甜高粱秸秆纤维素的降解效果，以5种不同发酵工艺所产的5组纤维素酶和商品纤维素酶为研究对象，通过对各纤维素酶的滤纸酶活、分解微晶纤维素所产的还原糖量及对甜高粱秸秆中纤维素转化率的检测可知，在5组自制的纤维素酶中，纤维素酶Ⅱ的滤纸酶活最高（1 364.84 U/mL），但纤维素酶Ⅴ对微晶纤维素的分解能力最强，并在50 ℃，48 h时其对甜高粱秸秆纤维素的转化率最高，达到11.69%，表明纤维素酶Ⅴ可高效分解甜高粱生物质材料转化成可发酵的糖，在发酵生物乙醇和高附加值的化工产品方面极具潜力。

Study of cellulase on highly effective degradation of sweet sorghum straw to sugars

To evaluate the effect of lab-made cellulase on sweet sorghum straw cellulose degradation, the filter paper activity (FPA) of cellulase, the reducing sugar content of microcrystalline cellulose (MCC) decomposition and the cellulose conversion rate of sweet sorghum straw were detected, respectively, with the 5 batches of cellulase produced by 5 different of fermentation processes and commercial cellulase as research object. The results showed that the FPA of cellulase Ⅱ was the highest (1 364.84 U/ml) in the 5 lab-made cellulase, which the decomposition capability of cellulase V on microcrystalline cellulose was the strongest, and its conversion rate was the highest on sweet sorghum straw cellulose at 50 ℃ for 48 h, reaching 11.69%. It indicated that cellulase V could effectively decompose sweet sorghum biomass materials into fermentable sugars, and had great potential for fermentation of bioethanol and high value-added chemical products.