不同产地蜂胶药材的HPLC指纹图谱

目的:通过建立蜂胶药材HPLC指纹图谱,评价不同产地蜂胶药材质量的差异,为蜂胶原料适宜产地的选择提供实验依据。方法:采用HPLC测定4个不同主产区14批不同产地蜂胶药材的指纹图谱,并采用国家药典委员会颁布的"中药色谱指纹图谱相似度评价系统"(2004 A)和聚类分析对指纹图谱进行相似度比较。结果:建立蜂胶药材指纹图谱共有模式,标定12个指纹图谱共有峰,14批蜂胶药材中9批蜂胶药材的相似度>0.90,说明不同主产区及同一主产区不同产地蜂胶指纹图谱既有共性又存在一定差异。结论:建立的方法精密度、重复性和稳定性较好,可用于蜂胶药材的的质量控制及产地鉴别。

HPLC Fingerprint Analysis of Propolis from Different Origins

Objective: By establishing HPLC fingerprint of propolis, to evaluate the quality differences of propolis from different areas, and provide experimental evidence for appropriate choice of raw propolis origins. Method: The fingerprints of 14 batches of propolis from four main producing areas were determined by HPLC method, and the fingerprints were compared for similarity by using "Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" (2004 A) issued by Chinese Pharmacopoeia commission and cluster analysis. Result: The common mode for propolis fingerprints was established, and 12 common fingerprint peaks were identified;the similarity was greater than 0.90 in 9 batches of the 14 batches of propolis medicinal herbs, indicating that propolis fingerprints had both similarities and differences either from different main producing areas or different fields within the same producing area. Conclusion: This method is reproducible, precise and steady, and it can be used for quality control and origin identification of propolis medicinal herbs.