微生物产氢机理的研究进展

微生物可以利用工业废弃物产生氢气,其产氢机理可以分成两种:光合产氢和发酵产氢。前者利用光能,后者利用代谢过程中产生的电子,分解有机物产氢。氢酶是产氢过程中的关键酶,催化氢的氧化或质子的还原。氢酶主要有NiFe]氢酶和Fe]氢酶两种,具有不同的结构,但催化机理是相似的。本文主要综述产氢微生物的种类、微生物产氢代谢途径和关键酶催化机理,并展望微生物产氢研究的发展方向。

Research advance in biohydrogen mechanism

Industrial waste can be reduced by microorganisms to produce hydrogen,its mechanism can be divided into two parts:biophotolysis and fermentation.Light is utilized by the former,while the organic compound and the electron produced in the metabolism are utilized by the latter.Hydrogenases are the key enzymes in the process of biohydrogen production,and catalyze the oxidation of hydrogen and the reduction of protons.NiFe] hydrogenase and Fe] hydrogenase are the main types of hydrogenases and have different structures but similar catalytic mechanism.This paper summarizes the types of hydrogen-producing microbes,the research on biohydrogen metabolism and the catalytic mechanism of hydrogenase,and brings forward the prospect.