不同状态胆囊组织的傅立叶变换红外光谱研究

目的 探索不同生理病理状态的胆囊组织各自特异的傅立叶变换红外光谱(Fourier transform infrared spectroscopy，FT-IR)表现。方法 应用FT-IR对8例正常胆囊组织、10例炎性胆囊组织和10例胆囊癌组织进行检测，结合常规病理结果总结不同组织的光谱特征。结果 胆囊正常、炎性和癌组织具有不同的FT-IR光谱表现，1550cm^-1处的酰胺Ⅱ带在癌组织中显得较弱，峰形低平，而在正常组织中则较强，峰形高尖。1080cm^-1处核酸的吸收谱带在癌组织中较强，I1080／I1550的比值在正常组织中为0．62，癌组织中为0．87。正常组织中1450cm^-1处的峰多强于1400cm^-1处，而在癌组织中则相反。结论 胆囊正常、炎性和癌组织的FT-UR谱图不同，主要表现为组织中蛋白质、核酸和磷脂等含量与结构的改变，FT-IR有望成为胆道疾病临床诊断的一种新手段。

A study on different tissues of gallbladder by Fourier transform infrared spectroscopy

Objective To investigate the special features of different physiological and pathological tissues of gallbladder by Fourier transform infrared spectroscopy (FT-IR). Methods Eight normal samples, 10 inflammatory samples and 10 cancer tissue samples of gallbladder were observed by FT-IR and the special spectrum data of different tissues were summarized. Results Different spectra were found in normal, inflammatory and cancer tissue samples. The peak at 1 550 cm-1 was weak and wide in cancer tissues but strong and high in normal tissues. The peak at 1 080 cm-1 was stronger in cancer tissues than in normal tissues. The ratio of I1080/I1550 was 0. 62 in normal tissues and 0. 87 in cancer tissues. The peak at 1 450 cm-1 was higher than 1 400 cm-1 in normal tissues and lower than 1 400 cm-1 in cancer tissues. Conclusions The normal, inflammatory and cancer tissues of gallbladder have different FT-IR spectra, which was mainly due to changes in protein, nucleic acid and phospho-lipid. FT-IR can be developed into a new method to diagnose biliary tract diseases.