Amino acid sequence surrounding the lipoic acid cofactor of bovine kidney 2-oxoglutarate dehydrogenase complex |
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| Authors: | A P Bradford A Aitken F Beg K G Cook S J Yeaman |
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| Affiliation: | Department of Biochemistry, University of Newcastle upon Tyne, England. |
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| Abstract: | The 2-oxoglutarate dehydrogenase complex was succinylated using 2-oxo5-14C]glutarate in the presence of N-ethylmaleimide to label the lipoic acid cofactor of the transuccinylase (E2) component. Following peptic digestion, 14C-lipoate-containing peptides were purified and subjected to automated Edman degradation and amino acid analysis. The amino acid sequence surrounding the lipoyllysine residue is reported. |
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