福氏痢疾菌膜成分与细菌抗原性和毒力的关系

应用免疫转移技术，用从感染福氏痢疾菌的病人获取的恢复期血清分析了福氏痢疾菌膜成分与细菌抗原性和毒力的关一。发现福氏痢疾菌的膜蛋白67kD和63kD均含有两种成分，一种和膜蛋白60kD都可能是保护性抗原，而另一种与膜蛋白78kD和35kD一样与福氏痢疾菌的毒力有关。采用微细胞同位素掺入示踪显示这些与病人恢复期血清反应的膜蛋白由质粒编码。

Membrane component of Shigella flexneri and their relationship to bacteria antigenicity and virulence

Our previous works have shown that membrane proteins of S. flexneri can elicit protection against the bacteria in vivo. In this study, we further, using human convalescent sera (HCS) from adult patients infected with S. flexneri, investigated the relationship between membrane components of S. flexneri and their antigenicities and virulences. It was shown in immunoblotting that 5 virulent strains of S. flexneri and avirulent strain T32 derived from S. flexneri 2a by Istrati in Romania and used as a live vaccine, had three common proteins bands formed by the components of MW 67 kD, 63 kD and 60 kD with either HCS from patients infected with S. flexneri 2a and 3a or HCS absorbed by an avirulent variant strain 24570 of S. flexneri 2a, while virulent strains M90T and M25-8A (pWR110)minI shown another two bands of Mw 78 kD and 35 kD respectively. In contrast, no band was found with strain 24570. HCS from patients infected with S. flexneri 1a, 2a and 3a as well as those HCS absorbed by 24570 had blocking effect on the invasiveness of virulent strain of S. flexneri to the kerato conjunctiva of guinea pigs; nevertheless, HCS absorbed by T32 lost the blocking effect accompanied by disappearance of 67 kD, 63 kD and 60 kD components when T32 was used as antigen indicating that 67 kD, 63 kD and 60 kD membrane components of S. flexneri may account for the antigen of S. flexneri eliciting protection antibodies in vivo.(ABSTRACT TRUNCATED AT 250 WORDS)