Isolation, identification and characterization of cadmium-resistant Pseudomonas aeruginosa strain E1 |
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Authors: | Xiao-xi Zeng Jian-xin Tang Xue-duan Liu and Pei Jiang |
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Affiliation: | (1) School of Resources Processing and Bioengineering, Central South University, Changsha, 410083, China;(2) College of Packaging and Printing, Hunan University of Technology, Zhuzhou, 410083, China |
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Abstract: | Strain E1 with resistance to 18 mmol/L cadmium (Cd), isolated from Cd-contaminated soil was identified by morphological observation,
biochemical and physiological characterization and 16S rDNA sequence analysis. The resistance to heavy metals Cd, Cu, Co,
Mn, Pb, Zn and 12 antibiotics was examined. The ability of removing Cd from solution was studied. The characterizations show
that strain E1 is affiliated to Pseudomonas aeruginosa (P. aeruginosa). Strain E1 has high resistance to heavy metals and the order is found to be Cd>Mn>Zn>Cu>Pb>Co in solid media. Strain E1 also exhibits the resistance to 12 antibiotics. Both living and non-living cells of strain E1 can remove Cd from solution, and living cell has better biosorption than non-living cell.
Foundation item: Project (50621063) supported by the National Natural Science Foundation of China; Project (2004CB619204) supported by the
Major State Basic Research and Development Program of China |
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Keywords: | identification P aeruginosa cadmium-resistance screening |
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