| Labeling of human insulin-like growth factor-I eukaryotic expression vector with green fluorescent protein |
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| 引用本文： | 刘一,张绍昆,吴宏Department of Ophthamology, Second Hospital, Jilin University, Changchun 130041, China,单玉兴,王刚,徐莘香. Labeling of human insulin-like growth factor-I eukaryotic expression vector with green fluorescent protein[J]. 中华创伤杂志(英文版), 2005, 8(3) |
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| 作者姓名： | 刘一 张绍昆 吴宏Department of Ophthamology Second Hospital Jilin University Changchun 130041 China 单玉兴 王刚 徐莘香 |
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| 作者单位： | Department of Orthopaedic Surgery，First Hospital，Jilin University，Changchun 130021，China，Department of Orthopaedic Surgery，First Hospital，Jilin University，Changchun 130021，China，Department of Orthopaedic Surgery，First Hospital，Jilin University，Changchun 130021，China，Department of Orthopaedic Surgery，First Hospital，Jilin University，Changchun 130021，China，Department of Orthopaedic Surgery，First Hospital，Jilin University，Changchun 130021，China |
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| 基金项目： | SupportedbyNationalNaturalScienceFoundationofChina(No.30400447)andbyfoundationforyoungteacherofJilin University(No.420010302361). |
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| 摘 要： | Articularcartilageinjuriesinadultsarecommon.Theintrinsicrepairisgenerallyminimalandcartilageinjuriescanfurtherdevelopintoosteoarthritis.Insulin likegrowthfactor I(IGF I)is regardedasoneofthemostimportantgrowthfactorsin cartilagedevelopmentandhomeostasis.Theadditionof IGF Itochondrocytesinvitroenhanceschondrocyte metabolism,maintainsadifferentiatedchondrocyte morphologyandpromotessynthesisofmajorcartilage matrixproteins,includingtype IIcollagenand proteoglycans.1,2IGF Iinlowconcentrationi…
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Labeling of human insulin-like growth factor-I eukaryotic expression vector with green fluorescent protein |
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| LIU Yi,ZHANG Shao-kun,WU Hong,SHAN Yu-xing,WANG Gang,XU Xin-Xiang. Labeling of human insulin-like growth factor-I eukaryotic expression vector with green fluorescent protein[J]. Chinese journal of traumatology, 2005, 8(3) |
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| Authors: | LIU Yi ZHANG Shao-kun WU Hong SHAN Yu-xing WANG Gang XU Xin-Xiang |
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| Abstract: | Objective: To label human insulin-like growth factor-I (hIGF-I) eukaryotic expression vector with green fluorescent protein (GFP) for the repair of articular cartilage defects. Methods: GFP cDNA was inserted into pcDNA_(3.1)-hIGF-1 to construct the co-expression vector with two multiple cloning sites mammalian expression vector under two cytomegalovirus promoters/enhancers respectively. Recombinant pcGI was transfected into NIH 3T3 cells with the help of lipofectamine. Results: Enzyme digestion and agarose gel electrophoresis analysis revealed that pcGI vector contained correct GFP and hIGF-I cDNA. Expression of (hIGF-1) and GFP was confirmed in transfected NIH 3T3 cells by immunocytochemical analysis and fluorescence microscopy. Conclusions: hIGF-I eukaryotic expression vector has been successfully labeled with GFP. |
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| Keywords: | Insulin-like growth factor I Transgenes Green fluorescent protein |
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