Immunity gene pedB enhances production of pediocin PA-1 in naturally resistant Lactococcus lactis strains |
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Authors: | Arqués J L Rodríguez J M Gasson M J Horn N |
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Affiliation: | * Commensals and Microflora, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom † Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain |
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Abstract: | Heterologous production of the antilisterial bacteriocin pediocin PA-1 in lactococci is an attractive objective to increase the safety of dairy products. In a previous paper, we developed a system for the heterologous production of the bacteriocin pediocin PA-1 in pediocin-resistant lactococcal hosts through a leader exchange strategy. The system was based on 3 genes, 1 encoding the fusion between the lactococcin A leader and propediocin PA-1, and the other 2 encoding the lactococcin A secretion machinery. In this study, we investigated whether the addition of the pediocin PA-1 immunity gene (pedB) to this system has any effect on pediocin production. Introduction of the plasmid(s) carrying the genes described above into nisinproducing and non-nisinproducing lactococcal hosts led to a significant increase in the production of pediocin compared with the equivalent pedB-devoid systems. In addition, we obtained a nisin-producing strain with the ability to secrete pediocin PA-1 at a level equivalent to that of the parental strain Pediococcus acidilactici 347, which represents a notable improvement over our previous systems. |
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Keywords: | Lactococcus lactis bacteriocin immunity heterologous production |
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