不同辐照模式聚焦超声诱导人胰腺癌细胞凋亡的量效关系

目的 探讨不同辐照模式聚焦超声诱导人胰腺癌细胞凋亡的最佳辐照方案。方法 对人胰腺癌细胞系PaTu 8988t细胞悬液进行聚焦超声辐照:第1组为空白对照组,第2、3组采用脉冲辐照模式,第4、5组采用连续辐照模式,各组辐照剂量通过改变辐照时间来改变。以流式细胞仪检测辐照后24 h肿瘤细胞凋亡率及细胞死亡率。结果 超声辐照后各组的细胞悬液最高温度分别为28.00℃、(42.20±2.17)℃、(50.80±0.84)℃、(55.80±2.17)℃、(65.20±3.11)℃,各组间差异均有统计学意义(P均<0.05)。各辐照组的细胞凋亡率为(0.56±0.15)%、(1.28±0.16)%、(1.84±0.29)%、(5.74±1.15)%、(2.00±0.84)%,细胞死亡率为(3.28±0.59)%、(4.60±0.47)%、(8.64±2.69)%、(41.12±15.91)%、(49.70±8.02)%,各组间差异均有统计学意义(P均<0.05)。结论 聚焦超声辐照剂量在接近热凝固温度阈值时,采用连续辐照模式可获得较高的细胞凋亡率及细胞死亡率。

Dose-effect of human pancreatic carcinoma cell apoptosis induced by focused ultrasound with different radiation patterns

Objective To explore the optimal radiation pattern of focused ultrasound induced pancreatic carcionoma cell apoptosis.Methods The human pancreatic carcinoma cell line PaTu 8988t suspension was radiated by focused ultrasound using 5 dose groups with different radiation time parameters and patterns.Pulsed radiation pattern was used in groups 2 and 3,while continuous radiation pattern was used in group 4 and 5,group 1 was taken as the controls.The ratio of focused ultrasound induced cell apoptosis and cell dea...