反义内皮素转换酶核酸对尘螨过敏哮喘患者外周血单个核细胞释放白细胞介素5的抑制作用

目的　探讨转导反义内皮素转换酶 (ECE)核酸的气道上皮细胞对尘螨过敏性患者外周血单个核细胞释放Th1/Th2相关细胞因子的影响。方法　尘螨过敏患者 2 1例 ,分离其外周血单个核细胞 (PBMC) ,根据实验要求分为 :未刺激组 (A组 )、反义ECE上皮细胞 +PBMCs组 (A1组 )、对照组上皮细胞 +PBMCs组 (A2 组 )及螨刺激组 (B组 )、反义ECE上皮细胞 +PBMCs+尘螨提取液组 (B1组 )、对照组上皮细胞 +PBMCs+尘螨提取液组 (B2 组 ) ,共培养 72h ,螨刺激组同时加入尘螨提取液 2 0 μg/ml。培养上清用酶联免疫吸附 (ELISA)法检测白细胞介素 5 (IL 5 )、γ干扰素 (IFN γ)。结果2 1例患者中有 12例患者经尘螨刺激后IL 5释放明显增加。将 12例患者进行统计学分析发现 ,未经尘螨刺激时 ,A1组IL 5和IFN γ水平分别为 (6 0± 1 3)× 10 -9g/L]和 (6 3± 2 6 )× 10 -9g/L],A2 组为 (7 5± 1 1)× 10 -9g/L]、(70± 5 2 )× 10 -9g/L],两组比较差异均无显著性 (P >0 0 5 ) ;尘螨刺激后 ,B1组的IL 5水平和IFN γ分别为 (8 2± 1 6 )× 10 -9g/L]、(10 0± 4 1)× 10 -9g/L],B2 组分别为(12 0± 1 8)× 10 -9g/L]、(15 3± 71)× 10 -9g/L],两组IL 5比较差异有显著性 (P =0 0 4 7) ,而IFN γ水平比较差异无显著性 (P >

Inhibition of antisense-endothelin converting enzyme RNA on interleukin-5 released from dust mite-challenged peripheral blood mononuclear cells in patients with allergic asthma

OBJECTIVE: To investigate the effect of antisense endothelin converting enzyme (ECE) RNA on levels of cytokines released from CD(4)(+) lymphocytes in patients with allergic diseases responsive to house dust mites. METHODS: Peripheral blood mononuclear cells (PBMCs) were separated from 21 patients who were sensitive to dust mites. PBMCs from those patients were divided into two groups. No stimulation group (A group) induded A(1) group (anti-ECE epithelial cells + PBMCs) and A(2) group (control cells + PBMCs). Stimulation group (B group) included B(1) group (anti-ECE epithelial cells + PBMCs + dust mites extract) and B(2) group (control cells + PBMCs + dust mites extract). House dust mite extract (20 microg/ml) was added to the culture of stimulation group as described above. After 72 hours, supernatants from both groups were collected and the levels of IL-5 and IFN-ggr; released into the supernatants were detected by enzyme-linked immunoabsorbent assay. RESULTS: IL-5 levels were increased significantly after treatment with dust mite in twelve of 21 cases. No significant differences of IL-5 were found between the groups of A(1)(6.0 +/- 1.3) x 10(-9) g/L] and A(2) (7.5 +/- 1.1) x 10(-9) g/L] before house dust mite stimulation in the 12 cases (P > 0.05), and no significant differences in IFN-ggr; were found between the groups of A(1) (63 +/- 26) x 10(-9) g/L] and A(2) (70 +/- 52) x 10(-9) g/L] before house dust mite stimulation (P > 0.05). IL-5 level was increased in both groups after stimulation but it was significantly lower in the B(1) group (8.2 +/- 1.6) x 10(-9) g/L] than that in the B(2) (12.0 +/- 1.8) x 10(-9) g/L] (P = 0.047). It seemed that increased IFN-ggr; level after stimulation was higher in B(2) (153 +/- 71) x 10(-9) g/L] than that in the B(1) group (100 +/- 41) x 10(-9) g/L], but there was no statistic significance (P > 0.05). In addition, our results also showed that the release of IL-5 was significantly increased in those cases with asthma, or asthma plus allergic rhinitis after dust mites stimulation (44 +/- 15)%] compared with that in those with urticaria (7 +/- 4)%] (P = 0.047). CONCLUSIONS: Antisense-ECE downregulated the IL-5 secretion from Th2 lymphocytes in patients with allergic asthma after being challenged with dust mites. It is indicated that ET-1 is an important cytokine involved with allergic airway inflammation. Antisense-ECE RNA management in airways may be of value in treating allergic asthma.