| 灰树花多糖的分离纯化及其体外抗肿瘤活性 |
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| 引用本文： | 陈沛,刘会平,孙娜新,刘子天,李委红,刘旭辉,刘少娟.灰树花多糖的分离纯化及其体外抗肿瘤活性[J].现代食品科技,2018,34(6):107-114. |
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| 作者姓名： | 陈沛 刘会平 孙娜新 刘子天 李委红 刘旭辉 刘少娟 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院 |
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| 摘 要： | 探究低温提取的灰树花多糖的结构及其体外抗肿瘤活性。利用低温(4℃)水提醇沉法提取灰树花粗多糖,采用Sevage法除去粗糖中的蛋白,透析除去盐类、寡糖等小分子物质,得到多糖组分命名为GFP,其得率为2.13%;离子色谱检测其主要单糖为葡萄糖、半乳糖和甘露糖;高效液相色谱分析结果表明GFP为混合物,含有两个主要的大分子群体,分子量分别为1700.00 ku和34.12ku,其组分峰面积分别为66.57%和28.23%;红外光谱显示GFP可能为α-吡喃多糖。GFP的抗肿瘤活性研究表明:在一定的浓度范围内,GFP能显著抑制人肝癌HepG2细胞的增殖;通过JC-1染色检测线粒体膜电势的变化发现GFP会引起线粒体膜电位降低,这说明GFP诱导的细胞凋亡伴随着膜电位的改变;经过GFP处理的HepG2细胞出现了典型的细胞凋亡的形态变化,细胞收缩,形成微核。以上均说明GFP诱导HepG2细胞凋亡。
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| 关 键 词： | 灰树花多糖 低温提取 抗肿瘤活性 |
| 收稿时间： | 2018/1/30 0:00:00 |
Isolation and Purification of Polysaccharide from Grifola frondosa and Its in vitro Antitumor Activity |
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| CHEN Pei,LIU Hui-ping,SUN Na-xin,LIU Zi-tian,LI Wei-hong,LIU Xu-hui and LIU Shao-juan.Isolation and Purification of Polysaccharide from Grifola frondosa and Its in vitro Antitumor Activity[J].Modern Food Science & Technology,2018,34(6):107-114. |
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| Authors: | CHEN Pei LIU Hui-ping SUN Na-xin LIU Zi-tian LI Wei-hong LIU Xu-hui and LIU Shao-juan |
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| Affiliation: | (Food Engineering and Biotechnology Institute, Tianjin University of Science and Technology School, Tianjin 300457, China),(Food Engineering and Biotechnology Institute, Tianjin University of Science and Technology School, Tianjin 300457, China),(Food Engineering and Biotechnology Institute, Tianjin University of Science and Technology School, Tianjin 300457, China),(Food Engineering and Biotechnology Institute, Tianjin University of Science and Technology School, Tianjin 300457, China),(Food Engineering and Biotechnology Institute, Tianjin University of Science and Technology School, Tianjin 300457, China),(Food Engineering and Biotechnology Institute, Tianjin University of Science and Technology School, Tianjin 300457, China) and (Food Engineering and Biotechnology Institute, Tianjin University of Science and Technology School, Tianjin 300457, China) |
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| Abstract: | |
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| Keywords: | Grifola frondosa polysaccharide extraction under low temperature antitumor activity |
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