MECC-AD法测定黄连和黄柏中小檗碱、巴马汀和药根碱的含量

建立检测黄连、黄柏中生物碱(小檗碱、巴马汀、药根碱)含量的MECC-AD测定方法。在自组装仪器上,以0.3 mm(i.d.)碳圆盘微电极为工作电极,在1.150 V(Ag/AgCl)的检测电位下,以含有0.008 mol·L-1的胆酸钠(SC)溶液与3%甲醇溶液的0.2mol·L-1H3BO3-0.05 mol·L-1Na2B4O7·10H2O缓冲液(pH值=8.45)为运行液;当毛细管长度65 cm(i.d.25μm),分离电压10 kV,三种待测物在15 min内完全分离。本方法测定三种生物碱的线性范围分别为(0.37~32.6、0.98~41.7、0.25~25.9μg·mL-1);检出限分别为(0.15、0.36、0.08μg·mL-1)。结论:该方法已成功应用于黄连、黄柏等中药材中3种生物碱含量的测定。

MECC-AD Determination of Berberine,Palmatine and Jatrorrhizine in Phellodendri Cortex and Coptidis Rhizoma

Micellar electrokinetic capillary chromatography （MECC） with amperometricl detection （AD） was used to determine three alkaloids （berberine, palmatine and jatrorrhizine） in Phellodendri Cortex and Coptidis Rhizoma. At the laboratory -built system a carbon disk electrode was used as the working electrode and the optimal detection potential was 1. 150 V （ versus Ag/AgCl）. At 10 kV of applied voltage and a 65cm （ i.d. 25 μm ） length capillary ,the three alkaloids were completely separated within 15 rain in 0.2 mol ·L^-1 HaBO3 - 0.05 mol ·L^-1 Na2B4O7 · 10H2O buffer （ pH = 8.45） including 0.008 mol ·L^-1 sodium cholate （SC） and 3% （v/v） methanol. The linear ranges for berberine, palmatine and jatrorrhizine are 0. 37 - 32.6, 0.98 - 41,7 and 0.25 - 25.9 μg·mL^-1; respectively, and the limit detections are 0.15, 0.36, 0. 08μg·mL^-11. This method has been successfully applied to the determination of the three alkaloids in Phellodendri Cortex and Coptidis Rhizoma.