氨肽酶N在结直肠癌中的表达及其临床意义

目的:检测结直肠癌中氨肽酶N(APN)的表达,并通过分析其与血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)的关系,探讨APN在结直肠癌发生、发展过程中的作用及其临床意义。方法:应用免疫组织化学技术,检测40例结直肠癌组织及40例癌旁正常直肠组织中APN、VEGF和bFGF的表达,并结合临床病例特征进行分析。结果:40例结肠癌组织中APN的阳性率达52.5%(21/40),15例转移淋巴组织中达66.67%(10/15),在结直肠癌组织和转移淋巴结组织中的阳性表达率均明显高于癌旁正常直肠组织,差异有统计学意义(P0.05)。APN、VEGF与bFGF在结直肠癌中的阳性表达均与患者的性别、年龄、肿瘤大小、肿瘤位置、分化程度无关(P0.05),而与肿瘤的分期和有无转移显著相关(P0.05)。结直肠癌组织中APN与VEGF、bFGF的表达间均呈显著正相关性(P0.05)。结论:APN在结直肠癌组织中呈高表达,与VEGF、bFGF可能具有协同作用,共同参与调节结直肠癌的发展,对于评估结直肠癌的生物学行为和预测患者的预后可能具有重要价值。

Expression and Clinical Significance of APN in Colorectal Carcinoma*

Objective： To investigate the expression and clinical significance of APN in colorectal carcinoma. Methods： Immunoh- istochemical technique was used to detect the expression of APN, VEGF and bFGF in 40 cases of colorectal carcinoma and 40 cases of normal colorectal mucosa, their correlation with the clinicopathological characteristics of colorectal carcinoma were analyzed. Results： The positive expression rates of APN were respectively 52.5%（21/40） in colorectal carcinoma and 66.67%（10/15） in metastasised lymph nodes, which were both significant higher than those in the adjacent normal tissues. The expression of APN, VEGF and bFGF were correlated with Dukes staging and lymph node metastasis （P〈0.05）, but had no correlation with gender, age, tumor size, tumor position, cell differentiation of colorectal carcinoma （P〉0.05）. The expression of APN was positively correlated with VEGF and bFGF（P〈0.01）. Conclusion： The expression of APN in colorectal cancer tissue was up-regulated, which mediated the development of colorectal cancer together with VEGF and bFGF and were of great importance in the estimation of biological behavior and prognosis ofcolorectal cancer.