利用核酶技术下调人胆管癌细胞bcl-2表达水平

目的 研究切割bcl-2mRNA核酶降低人胆管癌细胞bcl-2表达水平的作用。方法 将合成的切割bcl-2 mR—NA核酶基因与真核细胞表达载体重组，并用重组后的载体转染人胆管癌细胞，通过免疫细胞化学、流式细胞仪等手段，观察转染后的胆管癌细胞bcl-2的表达水平。结果 转染切割bcl-2mRNA核酶基因的胆管癌细胞内bcl-2的表达水平较对照组明显下降；部分细胞出现自发性凋亡现象。结论 切割bcl—2mRNA核酶可明显降低胆管癌细胞内bcl-2的表达水平，并能够诱导其出现自发性凋亡。

Down-regulation of the bcl-2 expression level in a human cholangiocarcinoma cell line using ribozyme

Objective To study the role of down regulation of bcl 2 expression of human cholangiocarcinoma cells with bcl 2 mRNA cleaving ribozyme (RZ). Methods Synthetic gene of bcl 2 mRNA cleaving RZ was recombined with the expression vector of eukaryotic cells. The recombined vector was used to transfect human cholangiocarcinoma cells. The expression of bcl 2 in human cholangiocarcinoma cells was observed by immunocytochemistry and flow cytometry. Results The expression of bcl 2 in human cholangiocarcinoma cells transfected with RZ gene was lower than that of control group. Spontaneous apoptosis was observed in a few of the cells. Conclusion bcl 2 mRNA cleaving RZ can down regulate the expression of bcl 2 of cholangiocarcinoma cells notably and can also induce spontaneous apoptosis.