一个棉花类受体蛋白激酶基因的克隆与表达分析

 利用简并引物扩增、染色体步移以及RT-PCR的方法，分离了棉花的一个类受体蛋白激酶基因的全长序列。序列分析表明，该基因没有内含子，开放读码框的长度为2964 bp，编码的多肽序列含有988个氨基酸并与拟南芥控制花器官脱落的基因HAESA-like2具有60%左右的相似性，将该基因命名为GRLK5。半定量RT-PCR分析发现GRLK5在种子、茎皮、根和纤维中的表达量较高，并且受ABA的诱导表达。将GRLK5整合到植物表达载体pCAMBIA2301中，构建植物过量表达载体，通过农杆菌介导，采用叶盘法转化烟草，PCR检测证明目的基因已经整合到烟草基因组中。

Molecular Cloning and Expression Analysis of a Receptor-like Protein Kinase Gene in Upland Cotton

The cDNA encoding the full length of a receptor-like protein kinase of cotton was cloned through degenerate primers amplification,genome walking and RT-PCR.Sequence analysis showed that introns were absent in the gene.It contains a 2964 bp open reading frame,encodes a deduced protein of 988 amino acid residues,and shows 60% homology to HAESA-like2,which regulates floral organ abscission in Arabidopsis.This gene was tentatively designated as GRLK5.GRLK5 expresses higher in seed,stem bark,root,and fibre than ...