胃腺癌中IGFⅠR的表达及与抑癌基因PTEN相关性的研究

目的:研究胰岛素样生长因子Ⅰ受体(IGFⅠR)与抑癌基因PTEN在胃腺癌组织中的表达,探讨IGFⅠR与PTEN的相关性及其与胃癌临床病理学特征的关系。方法:采用免疫组织化学SP法检测60例胃腺癌患者肿瘤组织和60例癌旁正常组织中IGFⅠR和PTEN的表达水平。结果:60例胃腺癌组织中IGFⅠR蛋白表达阳性率为70%(42/60),癌旁正常胃组织中的阳性表达率为35%(21/60),二者比较有显著差异(P=0.000);PTEN蛋白在胃腺癌中的阳性表达率为41.7%(25/60),在癌旁正常组织中的表达率为71.7%(43/60),两者比较有显著差异(P=0.001);IGFⅠR的表达水平与病人的年龄、性别无显著相关性(P>0.05),IGFⅠR在低分化腺癌表达要高于中高分化腺癌(P=0.002),有淋巴结转移者要高于无淋巴结转移者(P=0.003),并与肿瘤的临床分期(P=0.003)、浸润深度(P=0.006)有关;在胃腺癌组织中IGFⅠR和PTEN蛋白表达之间呈明显负相关(rs=-0.469,P<0.001)。结论:IGFⅠR的表达与胃癌的生物学行为密切相关,其高表达可能有助于肿瘤的生长和转移,IGFⅠR有可能成为一个很好的分子治疗靶点;抑癌基因PTEN的失活与胃癌的发生发展密切相关;PTEN可能对IGFⅠR的表达有着调控作用。

Expression of Insulin-Like Growth Factor Ⅰ Rreceptor and Its Correlation with the Tumor Suppressor Gene PTEN in Gastric Adenocarcinoma

Objective: To study the expression of insulin-like growth factor Ⅰ receptor(IGFⅠR) and tumor suppressor gene PTEN,and to investigate the relationship between IGFⅠR and PTEN and their correlation with clinical pathological characteristics in gastric adenocarcinoma.Methods: The expression of IGFⅠR and PTEN in 60 cases of the gastric adenocarcinoma and adjacent normal tissues were detected by the immunohistochemical SP technique.Results: The positive rate of IGFⅠR protein expression in gastric adenocarcinoma and adjacent normal tissues were 70.0%(42/60) and 35.0%(21/60) respectively with significant difference(P=0.000).The positive rate of PTEN protein expression was significantly different between gastric adenocarcinoma group(41.7%,25/60) and adjacent normal tissue group(71.7%,43/60).The expression of IGFⅠR had no correlation with age and sex.The expression of IGFⅠR was statistically higher in low differentiated adenocarcinoma than medium-high differentiated adenocarcinoma(P=0.002),and was significantly higher in gastric adenocarcinoma with metastasis in lymph node than those without metastasis in lymph node(P=0.003).IGFⅠR protein expression was positively related to the clinical stage(P=0.003) and the depth of tumor invasion(P=0.006).There was a significantly negative correlation between IGFⅠR and PTEN expression(rs=-0.469,P<0.001).Conclusion: IGFⅠR expression may be closely related to the biological behavior of gastric cancer.The high expression of IGFⅠR may contribute to the growth and metastasis of gastric cancer,and IGFⅠR is likely to become a molecular target of therapy in gastric cancer.Inactivation of the tumor suppressor gene PTEN is related to the development of gastric cancer.PTEN may regulate the expression of IGFⅠR.