Respiration-Based Measurement of Lung Deposition of a Fluorescent Dextrane Aerosol in a Marmoset Monkey |
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Authors: | Willem W. A. Bergers Dory van de Meent-van der Horst Marloes J. A. Joosen |
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Affiliation: | 1. TNO Prins Maurits Laboratory, Division Chemical and Biological Protection, Rijswijk, The Netherlandsbergers@pml.tno.nl;3. TNO Prins Maurits Laboratory, Division Chemical and Biological Protection, Rijswijk, The Netherlands |
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Abstract: | A technique for measurement of respiration-based lung deposition of an aerosol was investigated and subsequently applied in a pilot study with a marmoset monkey. The technique consisted of an aerosol exposure system for a marmoset using a face mask and a previously constructed monkey chair, a method for recovery of fluorescent dextrane from lung material, and respiration measurement of the marmoset by whole-body plethysmography. In the pilot study, a ketamine-anesthetized marmoset was exposed for 20 min to an FITC–dextrane aerosol atmosphere (200 μg/L air, particle size 1.5 μm mass median aerodynamic diameter [MMAD]). It was found that 3.4% of the inhaled aerosol was deposited in the lungs; the aerosol was distributed over the lung lobes with an higher concentration at the distal side. |
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Keywords: | Aging air pollution behavior blood pressure heart rate metabolic syndrome motor activity serum biomarker |
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