不同灸法对溃疡性结肠炎大鼠结肠黏膜黏蛋白调节作用的研究

目的：观察UC大鼠结肠损伤与黏蛋白分泌情况,探讨不同灸法对UC大鼠结肠损伤的修复作用。方法：60只SD大鼠随机数字表法分为正常组、模型组、隔药灸组、隔姜灸组、隔蒜灸组和温和灸组6组。采用免疫学方法,并加局部刺激制备实验性溃疡性结肠炎大鼠模型。隔药灸组、隔姜灸组、隔蒜灸组和温和灸组取天枢穴（双）、气海穴,分别施以相应的治疗,连续治疗14次。疗程结束后,分别观察各组动物的体重、大便、结肠大体形态及组织病理学改变、结肠黏膜黏蛋白分泌情况。结果：溃疡性结肠炎大鼠体重增长缓慢,出现腹泻症状;病理学观察可见溃疡形成;结肠黏膜中性黏蛋白和酸性黏蛋白分泌减少,与正常组比较具有显著差异（P〈0.01）。隔药灸组、隔姜灸组、隔蒜灸组、温和灸组经相应治疗后,大鼠体重增加,腹泻症状得到明显改善,结肠肠壁各层结构明显改善。除温和灸组溃疡面尚未愈合外,隔药灸组、隔姜灸组、隔蒜灸组溃疡面已经基本愈合。结肠黏膜中性黏蛋白和酸性黏蛋白分泌上调,与模型组比较具有显著差异（P〈0.05～0.01）。结论：隔药灸、隔姜灸、隔蒜灸、温和灸可以通过调节黏蛋白表达,起到修复UC大鼠结肠损伤的作用。

The Regulating Effect on Different Moxibustion for Colonic Mucosa Mucin in Rats with Ulcerative Colitis

Objective：To investigate the injury of colon and the secretion of mucin in rats of ulcerative colitis treated by different moxibustion. Methods：60 rats were randomly assigned to normal,model,herb-partition moxibstion,ginger-partition moxibustion,garlic-partition moxibustion and warm moxibustion groups. A rat model of UC was established by an immunological method plus local stimulation. Points bilateral Tianshu and Qihai were selected for herb-partition moxibstion,ginger-partition moxibustion,garlic-partition moxibustion and warm moxibustion groups,the treatment continued for 14 times. After treatment,the weight,stool,general form and morphology changes of colonic tissues were observed. The secretion of mucin was examined. Results：The weight of model rats increased slowly,and the diarrhea sign was occur,the ulcer were observed in pathology;the neutral and acor mucin excretion were significantly reduced compared with normal group （P〈0.01）.The weight,stool system and colon structure of rats were improver by the treatment of herb-partition moxibstion ,ginger-partition moxibustion,garlic-partition moxibustion and warm moxibustion.Apart of the warm moxibustion group,the ulcer almost have been healed in herb-partition moxibstion group,ginger-partition moxibustion group,garlic-partition moxibustion group. The excretion of neutral and acor mucin were up-regulated compared with model group in colon mucosa （P〈0.05-0.01）. Conclusion：The injured colon mucosa can be recoved by herb-partition moxibstion ,ginger-partition moxibustion,garlic-partition moxibustion and warm moxibustion,through by regulating the mucin expression.