| 硫酸蒽酮法与硫酸苯酚法测定灵芝多糖含量比较 |
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| 引用本文： | 郭晓蕾,朱思潮,翟旭峰,王怀豫,宝丽.硫酸蒽酮法与硫酸苯酚法测定灵芝多糖含量比较[J].中医药学刊,2010(9):2000-2002. |
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| 作者姓名： | 郭晓蕾 朱思潮 翟旭峰 王怀豫 宝丽 |
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| 作者单位： | [1]无限极（中国）有限公司,广东广州510665 [2]中国科学院微生物研究所真菌地衣系统学实验室,北京100101 |
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| 摘 要： | 目的：比较硫酸蒽酮法与硫酸苯酚法测定灵芝多糖含量的差异。方法：分别采用上述两种方法利用紫外可见分光光度计测定灵芝中多糖的含量。结果：利用上述两种方法分别测定同一样品灵芝多糖的含量,硫酸蒽酮法测定结果高于硫酸苯酚法。结论：两种方法均操作简便,稳定可行,较之硫酸苯酚法,药典记载的硫酸蒽酮法更加科学合理。
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| 关 键 词： | 灵芝多糖 硫酸蒽酮法 硫酸苯酚法 含量测定 |
Comparison of Methods in Determination of Polysaccharide in Ganoderma Lucidum |
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| GUO Xiao-lei,ZHU Si-chao,ZHAI Xu-feng,WANG Huai-yu,BAO Li.Comparison of Methods in Determination of Polysaccharide in Ganoderma Lucidum[J].Study Journal of Traditional Chinese Medicine,2010(9):2000-2002. |
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| Authors: | GUO Xiao-lei ZHU Si-chao ZHAI Xu-feng WANG Huai-yu BAO Li |
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| Affiliation: | 1.Infinitus(china) Company Ltd,Guangzhou 510665,Guangdong,China;2.Key Lab of Systematic Mycology and Lichenology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China) |
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| Abstract: | Objective：To compare difference in determination content between phenol-vitriolic colorimetry method and anthrone sulphuric acid colorimetry method for polysaccharides in Ganoderma lucidum.Methods：Above two methods were employed to determine content of polysaccharides by UV spectrophotography. Results：The determination value of anthrone-sulphuric acid colorimetry method is high than the phenol-vitriolic colorimetry method.Conclusion：The result of anthrone-sulphuric acid colorimetry method is more reliable and accurate,it can be considered as the optimum assay method for polysaccharides in Ganoderma lucidum. |
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| Keywords: | Ganoderma lucidum polysaccharide anthrone sulphuric acid colorimetry phenol-vitriolic colorimetry content determination |
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