| 蔓长春花的组织培养与快速繁殖体系的建立 |
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| 引用本文： | 崔丽,晏升禄,徐爽,李祖任,廖海民. 蔓长春花的组织培养与快速繁殖体系的建立[J]. 贵州农业科学, 2012, 40(7): 48-50 |
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| 作者姓名： | 崔丽 晏升禄 徐爽 李祖任 廖海民 |
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| 作者单位： | 贵州大学生命科学学院,贵州贵阳,550025 |
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| 基金项目： | 贵阳市重大科技专项攻关“‘两湖一库’典型地带植被恢复关键技术研究与示范” |
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| 摘 要： | 为了建立蔓长春花的组培快繁体系,以蔓长春花的茎段为外植体,在添加不同植物激素的培养基中进行蔓长春花茎段的诱导和植株再生培养研究.结果表明:丛生芽增殖的最佳培养基为MS+2.0mg/L 6-BA+0.1～0.5mg/L NAA,试管苗的最佳生根培养基为1/2 MS+0.8～1.2mg/L IBA+2％蔗糖.
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| 关 键 词： | 蔓长春花 茎段 组织培养 快速繁殖 |
Tissue Culture and Establishment of Rapid Propagation System of Vinca major |
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| CUI Li , YAN Sheng-lu , XU Shuang , LI Zu-ren , LIAO Hai-min. Tissue Culture and Establishment of Rapid Propagation System of Vinca major[J]. Guizhou Agricultural Sciences, 2012, 40(7): 48-50 |
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| Authors: | CUI Li YAN Sheng-lu XU Shuang LI Zu-ren LIAO Hai-min |
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| Affiliation: | (College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025,China) |
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| Abstract: | To establish the rapid propagation system of V.major,the induction and plant regeneration of V.major stems were studied on culture media with different plant hormones taking the stem of V.major as the explant.The results showed that the optimum culture medium for cluster buds propagation was MS + 2.0 mg/L 6-BA + 0.1~0.5 mg/L NAA,and the best culture medium for rooting of test-tube plantlets was 1/2 MS+0.8~1.2 mg/L IBA+2% sucrose. |
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| Keywords: | Vinca major stem tissue culture rapid propagation |
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