米诺环素对体外培养的成年大鼠牙槽骨成骨细胞生物活性的影响

目的:研究不同浓度的米诺环素对体外培养的成年大鼠牙槽骨成骨细胞(mature rat alveolarosteoblast,MRAOBs)增殖、蛋白合成及碱性磷酸酶活性(alkaline phosphatase,ALP)的影响。方法:将不同浓度的米诺环素(1、5、10、20、40、100、200mg/L)加入体外培养的第4代MRAOBs中,孵育2d和4d后,倒置显微镜下观察其对细胞形态的影响,并于2d后检测其对细胞的增殖活性及蛋白合成的影响,4d后检测其对细胞的碱性磷酸酶活性的影响。结果:在1-200mg/L的浓度范围内,米诺环素对细胞形态无影响,可显著促进MRAOB的增殖和蛋白合成(P<0.01),1-100mg/L浓度范围内随着浓度的增加,促进细胞增殖和蛋白合成的作用显著增强,至100mg/L时达最大促进效应。1-200mg/L的米诺环素能显著抑制MRAOBs的ALP活性,随浓度的增加抑制作用逐渐增强。结论:一定浓度的米诺环素有促进成骨细胞的增殖、蛋白合成和抑制成骨细胞分化的作用。

Biological effects of minocycline on cultured mature rat alveolar osteoblasts in vitro

Objective: To investigate the influences of minocycline on the proliferation, protein synthesis and alkaline phosphatase (ALP) activity of mature rat alveolar osteoblasts (MRAOBs). Methods: Minocycline of Various concentrations (1, 5, 10, 20, 40, 100, 200mg/L) were added to the medium of cultured MRAOBs respectively. After co-incubated for 2 days cell proliferation activity was assayed using MTT, and the total amount of protein was detected with Coumassie Bright Blue method. After 4 days of co-incubation, the ALP activity was measured. Results: As the minocycline concentration increased within 1-100mg/L, the effects of promoting proliferative activity and protein synthesis were enhanced. The maximum promotion was obtained at 100mg/L. However, at a concentration range of 1 to 200mg/L, minocycline significantly inhibited cellular ALP activity. And the inhibition was enlcmced with gradually imcreased concentration of minocycline. Conclusion: The results suggest that proper doses of minocycline could promote cell proliferation and protein synthesis, inhibit cell ALP activity.