姜黄素通过增强自噬水平抑制急性肺损伤大鼠肺组织炎症反应

目的 观察脂多糖(LPS)诱导的急性肺损伤(ALI)大鼠模型肺组织中自噬表达,探讨姜黄素是否通过上调急性肺损伤大鼠自噬水平抑制肺组织的炎症反应.方法 40只雄性SD大鼠随机分为对照组、急性肺损伤组(ALI组)、姜黄素组、自噬抑制剂3-甲基腺嘌呤组(3-MA组).采用一次性气管内滴注LPS(4 mg/kg)制备ALI大鼠...

Curcumin inhibiting inflammation in lung tissue via enhancing autophagy level after acute lung injury in rats

To investigate whether curcumin inhibits the inflammatory response of lung tissues through upregulating the level of autophagy in rats with acute lung injury through assessing the autophagy level in the lung tissue in lipopolysaccharide(LPS)-induced acute lung injury(ALI) model. Methods Forty male SD rats were randomly divided into control group, ALI group, curcumin group and autophagy inhibitor 3-methyladenine group(3-MA group). The ALI rat model was prepared using a single intratracheal drip of LPS(4 mg/kg). Curcumin(200 mg/kg) or autophagy inhibitor 3-MA(15 mg/kg) was injected intraperitoneally into the curcumin and 3-MA groups respectively 15 min before modeling. The morphological changes of lung tissues were observed by HE staining at 24 h post-modeling. ELISA analysis was engaged to detect the total number of cells and the content of TNF-α, IL-1β and IL-6 in the BALF. The mRNA levels of ATG5 and ATG7 were measured with Real-time PCR. Autophagy marker proteins microtubule-associated protein 1 light chain 3(LC-3) and Beclin-1 were measured by Western blot. Results Compared with control group, the ALI group showed a large infiltration of inflammatory cells in the lung tissue, an increase in the total number of cells in the BALF, a significant upregulation of TNF-α,IL-1β and IL-6 levels compared with the control group(P<0.05), and a notable upregulation of autophagy indexes ATG5 and ATG7 mRNA levels and LC-3 and Beclin-1 protein expression(P<0.05). Compared with the ALI group, the curcumin group showed less infiltration of inflammatory cells, decreased total number of cells in BALF, significantly reduced levels of TNF-α,IL-1β and IL-6(P<0.05), and further upregulation of the expression of autophagy-related genes and proteins(P<0.05). 3-MA treatment significantly inhibited the expression of autophagy-related indicators, aggravated the infiltration of inflammatory cells,and increased the levels of TNF-α and IL-1β in BALF(P < 0.05). Conclusion Curcumin could provide the protective effects against LPS-induced inflammatory injury through enhancing autophagy activation.