| 两种高效液相色谱法测定茶叶中茶氨酸含量的比较 |
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| 引用本文： | 杨卫,鲜殊,王玺,李大祥,宛晓春.两种高效液相色谱法测定茶叶中茶氨酸含量的比较[J].食品与发酵工业,2011,37(6). |
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| 作者姓名： | 杨卫 鲜殊 王玺 李大祥 宛晓春 |
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| 作者单位： | 安徽农业大学农业部茶及药用植物安全生产重点开放实验室,安徽合肥,230036 |
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| 基金项目： | 现代农业(茶叶)产业技术体系建设专项资金(农科教发[2008]10号); 国家标准化工作项目资助 |
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| 摘 要： | 选取白茶为原料,比较了2种HPLC方法测定茶叶中茶氨酸含量,即不经衍生的三氟乙酸洗脱法和经邻苯二甲醛(OPA)柱前衍生的国标法(GB/T 23193-2008)在茶氨酸含量测定上的数值差异。结果显示:以Wa-ters AccQ.Tag化学试剂包的测定结果作为参照,三氟乙酸洗脱法测定结果与AccQ-Tag试剂盒法的测定结果比较接近,而GB/T 23193-2008测定结果与AccQ-Tag试剂盒测定结果在5%的显著水平上差异显著。在此基础上,对GB/T 23193-2008法中的OPA衍生化反应时间进行了实验论证,发现在338 nm检测波长下,衍生化产物在衍生后15～20 min内最稳定且具有最大信号响应值。
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| 关 键 词： | 茶氨酸 HPLC 方法比较 |
The Comparative Study on Theanine Quantitation by Two High-performance Liquid Chromatography Methods |
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| Yang Wei,Xian Shu,Wang Xi,Li Da-xiang,Wan Xiao-chun.The Comparative Study on Theanine Quantitation by Two High-performance Liquid Chromatography Methods[J].Food and Fermentation Industries,2011,37(6). |
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| Authors: | Yang Wei Xian Shu Wang Xi Li Da-xiang Wan Xiao-chun |
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| Affiliation: | Yang Wei,Xian Shu,Wang Xi,Li Da-xiang,Wan Xiao-chun(Key Laboratory of Tea & Medicinal Plant and Product Safety of Ministry of Agriculture,Anhui Agricultural University,Hefei 230036,China) |
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| Abstract: | In this paper,HPLC eluting with trifluoroacetic acid(TFA) solution for theanine determination in white tea was compared to the national standard HPLC method(GB/T 23193-2008) with the pre-column derivation by o-phthaldialdehyde(OPA).The reference was Waters AccQ-Tag Kit.The results showed that theanine contents detected by HPLC method with TFA elution are more close to Waters AccQ-Tag kit,while GB/T 23293-2008 method had significant differences in a significant level of 5% compared to Waters AccQ-Tag kit.Fur... |
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| Keywords: | theanine HPLC method comparison |
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