| 基于大孔树脂吸附的樟芝胞外总三萜的分离工艺 |
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| 引用本文： | 陆震鸣,许泓瑜,史劲松,许正宏. 基于大孔树脂吸附的樟芝胞外总三萜的分离工艺[J]. 食品与发酵工业, 2011, 37(6) |
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| 作者姓名： | 陆震鸣 许泓瑜 史劲松 许正宏 |
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| 作者单位： | 1. 江南大学医药学院制药工程研究室,江苏无锡,214122
2. 江南大学医药学院生物活性制品加工工程研究室,江苏无锡,214122 |
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| 基金项目： | 江苏省自然科学基金(BK2010142); 教育部新世纪人才支持计划(No.NCET-07-0380); 国家“863”重点项目子课题(No.2007AA021506) |
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| 摘 要： | 以总三萜的洗脱效果为考察指标,研究了大孔树脂分离纯化的吸附性能及洗脱参数。结果表明,XAD-16大孔树脂适宜樟芝胞外总三萜的富集分离,其再生能力良好,吸附过程符合Langmuir方程。同时获得了XAD-16大孔树脂对三萜类化合物的动态分离条件:上样发酵液的pH为6.0,吸附流速为2 mL/min,解吸附溶剂为乙醇。溶液中三萜类化合物的初始浓度为0.97 mg/mL,获得了纯度为260.54 mg/g的总三萜样品。
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| 关 键 词： | 大孔树脂 总三萜 樟芝 |
Separation of Total Triterpenoids from Culture Broth of Antrodia camphorata Based on Macroporous Resin Adsorption |
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| Lu Zhen-ming,Xu Hong-yu,Shi Jing-song,Xu Zheng-hong. Separation of Total Triterpenoids from Culture Broth of Antrodia camphorata Based on Macroporous Resin Adsorption[J]. Food and Fermentation Industries, 2011, 37(6) |
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| Authors: | Lu Zhen-ming Xu Hong-yu Shi Jing-song Xu Zheng-hong |
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| Affiliation: | Lu Zhen-ming1,Xu Hong-yu1,Shi Jing-song2,Xu Zheng-hong1 1(Laboratory of Pharmaceutical Engineering,School of Medicine and Pharmaceutics,Jiangnan University,Wuxi 214122,China) 2(Laboratory of Bioactive Products Process Engineering,China) |
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| Abstract: | Based on desorption effect on total triterpenoids in culture broth of Antrodia camphorata,the adsorption capability and desorption parameters of macroporous resin were studied.The best macroporous resin,XAD-16,has good regeneration ability and the adsorption process is in accordance with Langmuir equation.The optimal separation conditions were as follows: culture broth with pH of 6.0,flow rate of 2 mL/min,100% Ethanol as desorption solution.After separation by macroporous resin,total triterpenoids sample wi... |
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| Keywords: | macroporous resins total triterpenoid Antrodia camphorata |
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