应用日本血吸虫病患者血清特异性抗体亚类考核疗效

用纯化的日本血吸虫成虫３１／３２ＫＤ原建立的ＢＡ－ＥＬＩＳＡ系统检测慢性血吸虫病患者治疗前、后血清中的ＩｇＧ１，ＩｇＧ３和ＩｇＧ４抗体水平，同时用常规ＥＬＩＳＡ检测ＩｇＧ和ＩｇＭ特异抗体。结果表明：各类抗体均未见假阳性，亦未与肝吸虫病和肺吸虫病者血清出现交叉阳性。提示ＩｇＧ１和ＩｇＧ４抗体的敏感性和特异性高，具有诊断和疗效考核的价值。

Detection of different class (subclass) antibodies in sera of patients with schistosomiasis japonica for diagnosis and efficiency evaluation]

The subclass antibodies against IgG1, IgG3 and IgG4 in sera of the patients with chronic Schistosomiasis japonica were detected before treatment, and after treatment--6 and 12 months respectively, using Biotin-Avidin-ELISA (BA-ELISA) established by purified 31/32 KD antigen from the adult worms. At the same time IgG1 and IgM were examined by the standard ELISA. False positive reaction with normal control and cross reaction with other parasitic diseases have not been observed. The IgG1 and IgG3 subclasses showed high sensitivity and specificity and reduced quickly 6 months after treatment. These results indicate that the level of specific IgG1 and IgG4 to the 31/32 KD adult worm protein has high value for diagnosis of Schistosomiasis japonica and evaluation of the curative efficiency of the disease.