子宫颈鳞状细胞癌组织中细胞凋亡及相关调控基因蛋白的表达

目的：通过对细胞凋亡及部分相关蛋白表达进行检测，探讨子宫颈鳞状细胞癌组织中凋亡细胞的调控机制。方法：应用原位末端标记法（ＴＵＮＥＬ）和双重免疫荧光染色技术对正常子宫颈及不同分化程度的鳞状细胞癌组织中凋亡细胞和Ｂａｘ、Ｂｃｌ－２、ｃａｓｐａｓｅ－３蛋白表达进行检测，利用共聚焦显微镜观察结果。结果：ＴＵＮＥＬ法检测发现，每例标本均有不同程度的细胞凋亡，但数量及分布区域不完全相同，低分化鳞状细胞癌凋亡细

Expression of apoptosis and the related proteins in cervical carcinoma

Objective: To explore the mechanisms of apoptosis in cervical squamous cell carcinoma. Method: Immunohistochemical observations including TDT/dUTP nick end labeling (TUNEL) method and Dual fluorescent labeling techniques performed on cervical normal and squamous cell carcinoma tissues to detect apoptosis and expression of caspase-3, Bax and Bcl-2 protein, and the fluorescent results were detected by a confocal laser scanning microscopy. Results:The apoptosis and caspase-3 protein expressed in each case,and the amount and distribution of caspase-3 positive cells were same as apoptitic cells. Immunoreactivitives for Bax increased considerably in the cervical squamous cell carcinoma tissue. The Bax positive cells were fewer than caspase-3. Double staining for apoptosis and caspase-3 or Bax demonstrated in some cells. NO significant differences were observed in cervical tumor tissueas compared with controls for Bcl-2. Conclusion: Variant apoptosis appeared in cervical normal and Squamous cell carcinoma tissues. The expression of casapse-3 and increase of Bax/Bcl-2 were related to apoptosis. The apoptotic mechanism of different kind of cells are not same.