分泌抗细粒棘球绦虫单克隆抗体杂交瘤细胞株的建立

为了对包虫病的免疫诊断和免疫预防提供有价值的单克隆抗体,通过对骨髓瘤细胞SP2/0与用细粒棘球蚴囊液免疫的BALB/c小鼠脾细胞融合试验及多次筛选、克隆,建立了分泌单克降抗体的细胞株NIA_2。用ELISA及免疫电镜等方法检测证实该细胞株有特异性。其培养上清分泌抗体滴度为1:2560,注入同系小鼠腹腔,诱生肿瘤和滴度为1:163840的腹水。免疫球蛋白鉴定表明是IgG_1,未见对囊虫的阳性反应。该杂交瘤细胞株经组织培养传代10个月,分泌抗细粒棘球绦虫抗体性能稳定。

Cell line of hybridoma secreting monoclonal antibodies against Echinococcus granulosus antigens

In order to produce specific monoclonal antibodies for immunodiagnosis and immunoprophylaxis in hydatid disease, a monoclonal antibody cell line NIA2 secreting specific antibodies was established, through fusion of myeloma cells SP2/0 and spleen cells of BALB/c mice immunized with hydatid fluid from a local (Ningxia) patient and repeated screenings and clonings. The specificity was assayed via ELISA, immunoprecipitating and ultramicroscopy. Antibody titer of 1:2,560 was obtained in the supernatant of the culture medium by ELISA and a high titer of 1:163,840 in seeded ascitic fluid of mice of the same isolate. Immunoglobulin subclass of the NIA2 secreting antibody was determined to be IgG1 which showed no cross reaction to Cysticercus cellulosae. Cloned cell lines from the hybridomas were found consistent in secreting anti-Echinococcus granulosus antibody through serial passage in tissue culture for ten months.