| 山东小麦品种中高分子量麦谷蛋白1Bx14亚基特异PCR标记 |
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| 引用本文： | 许淼,李庭铂,王宪泽.山东小麦品种中高分子量麦谷蛋白1Bx14亚基特异PCR标记[J].山东农业科学,2005(3):6-8. |
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| 作者姓名： | 许淼 李庭铂 王宪泽 |
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| 作者单位： | 1. 山东农业大学生命科学学院,山东,泰安,271018
2. 聊城市东昌府区农业局,山东,聊城,252000 |
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| 基金项目： | 山东省"三О"工程项目 |
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| 摘 要： | 根据已知小麦1Bx14基因序列设计一对特异引物,对HMW -GS在Glu -Bx14位点已知的7个小麦品种进行了PCR扩增,结果表明,凡具有1Bx14的品种都能扩增出4 19bp的特异性带,与SDS -PAGE电泳结果一致。利用这一特异性标记对山东推广的2 0个小麦品种进行检测,发现7个品种带有1Bx14基因。研究表明,与SDS -PAGE相比,PCR标记更加快速、简便和准确。
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| 关 键 词： | 小麦 高分子量麦谷蛋白1Bx14亚基 PCR标记 |
| 文章编号： | 1001-4942(2005)03-0006-03 |
| 修稿时间： | 2004年11月30 |
Marking of high molecular glutenin 1Bx14 subunit using specific PCR marker in Shandong winter wheat cultivars |
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| XU Miao,LI Ting-bo,WANG Xian-ze.Marking of high molecular glutenin 1Bx14 subunit using specific PCR marker in Shandong winter wheat cultivars[J].Shandong Agricultural Sciences,2005(3):6-8. |
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| Authors: | XU Miao LI Ting-bo WANG Xian-ze |
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| Abstract: | A pair of specific primers designed based on the known wheat 1Bx14 gene sequence were applied to amplify 7 wheat cultivars with known HMW-GS in Glu-Bx14 locus. The results indicated that 419bp specific bands were appeared in all wheat cultivars amplified with 1Bx14, which was in coincidence with the results of SDS-PAGE. 20 wheat cultivars in Shandong province were screened applying the specific marker, and 7 cultivars were found out with 1Bx14 gene. Compared with SDS-PAGE, PCR marker was more rapid, handy and exact. |
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| Keywords: | Wheat High molecular glutenin Subunit PCR |
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