| 胃癌及癌旁正常组织中微小RNA表达谱的检测 |
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| 引用本文： | 陆俊,宋军,徐溢新,孟松,白津,郑骏年,徐为.胃癌及癌旁正常组织中微小RNA表达谱的检测[J].中华实验外科杂志,2011,28(8). |
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| 作者姓名： | 陆俊 宋军 徐溢新 孟松 白津 郑骏年 徐为 |
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| 作者单位： | 1. 徐州医学院附属医院普外科,江苏,221002
2. 江苏省肿瘤生物治疗重点实验室 |
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| 基金项目： | 徐州市科技发展基金资助项目 |
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| 摘 要： | 目的 应用微小RNA(miRNA)芯片技术筛选胃癌及癌旁正常组织中差异表达的miRNAs,从中发现与胃癌发生发展相关的miRNAs.方法 收集14例新鲜胃癌组织及癌旁正常黏膜组织,从标本中分离出小RNA,并用Cy3、Cy5双色荧光标记,将标记的小RNA在miRNAs芯片上进行杂交反应.结果 软件MeV4.0对芯片数据进行聚类分析,筛选获得92个在胃癌及癌旁正常组织中差异表达的miRNAs(P＜0.05).相对于癌旁组织,在胃癌组织中有8个miRNAs表达显著上调,11个miRNAs表达显著下调(P＜0.01).结论 胃癌组织和癌旁正常组织中存在差异表达的miRNA分子,它可能与胃癌的发生发展有关.Abstract：Objective To screen and identify the microRNA (miRNA) differential expression profiles in gastric cancer and paired adjacent normal tissue by the miRNA microarray technique. Methods Forteen gastric cancer fresh tissue samples and paired adjacent normal mucosa tissue samples were collected. Small RNA was isolated from the samples, labeled with Cy3, Cy5 two-color fluorescence and hybridized on miRNA microarray. Results By analysis of Software MeV4. 0 based on microarrays screening, 92 gastric cancer related miRNAs (P ＜ 0. 05 ) were obtained. In the gastric carcinoma, compared with paired adjacent normal tissue, 8 miRNAs were significantly up-regulated and 11 miRNAs were significantly downregulated (P ＜ 0. 01 ). Conclusion MiRNAs are differentially expressed between gastric carcinoma and adjacent normal tissue, which may be related to the pathogenesis and progression of gastric cancer.
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| 关 键 词： | 微小RNA 胃癌 表达差异 |
Detection and analysis of microRNA expression profiles of gastric cancer and adjacent normal tissues |
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| LU Jun,SONG Jun,XU Yi-xin,MENG Song,BAI Jin,ZHENG Jun-nian,XU Wei.Detection and analysis of microRNA expression profiles of gastric cancer and adjacent normal tissues[J].Chinese Journal of Experimental Surgery,2011,28(8). |
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| Authors: | LU Jun SONG Jun XU Yi-xin MENG Song BAI Jin ZHENG Jun-nian XU Wei |
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| Abstract: | Objective To screen and identify the microRNA (miRNA) differential expression profiles in gastric cancer and paired adjacent normal tissue by the miRNA microarray technique. Methods Forteen gastric cancer fresh tissue samples and paired adjacent normal mucosa tissue samples were collected. Small RNA was isolated from the samples, labeled with Cy3, Cy5 two-color fluorescence and hybridized on miRNA microarray. Results By analysis of Software MeV4. 0 based on microarrays screening, 92 gastric cancer related miRNAs (P ＜ 0. 05 ) were obtained. In the gastric carcinoma, compared with paired adjacent normal tissue, 8 miRNAs were significantly up-regulated and 11 miRNAs were significantly downregulated (P ＜ 0. 01 ). Conclusion MiRNAs are differentially expressed between gastric carcinoma and adjacent normal tissue, which may be related to the pathogenesis and progression of gastric cancer. |
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| Keywords: | MicroRNA Gastric carcinoma Differential expression |
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