RP-HPLC测定金刚藤中薯蓣皂苷元的含量

 目的　建立金刚藤中薯蓣皂苷元的测定方法。方法　采用RP-HPLC测定薯蓣皂苷元的含量,色谱柱为Lichrospher C₁₈(4.6mm×250mm,5μm),流动相为甲醇,检测波长为210nm,峰面积外标法定量。采用正交设计,考察发酵时间(A)、水解时间(B)、硫酸浓度(C)3个因素对薯蓣皂苷水解的影响。结果　薯蓣皂苷元在50～350μg·mL^-1内具有良好的线性关系(r=0.9991,n=7) ,平均回收率为99.97%,RSD=0.85%(n=5 )。采用发酵72h后用浓度为5%的硫酸水解6h,可使金刚藤中薯蓣皂苷水解完全。结论　RP-HPLC是一个简便、快速、分离效果好、精密度高、准确度好的测定金刚藤中薯蓣皂苷元的方法。发酵对水解产率影响显著。

RP-HPLC determination of diosgenin in Smilax china L.

OBJECTIVE To establish a method for determining diosgenin in Smilax china L.METHODS A Lichrospher C₁₈ (4.6mm×250mm,5μm) column was used with methand as the mobile phase.The detection wavelength was at 210 nm An external standardization method was used.Three factors on dioscin hydrolysis,I.e.zymolysis time(A), hydrolysis time (B), the concentration of sulfuric acid? were studied by orthogoral design.RESULTS The linear range of 50～350 μg·mL^-1 (r=0.9991,n=7)and an average recovery of 99.97%,RSD=0.85%(n=5)were obtained.The optimum hydrolytic condition included zymolysis for 72 h and hydrolysis for 6 h with 5% sulfuric acid.CONCLUSION RP-HPLC is a simple, rapid and reliable method for diosgenin determination in Smilax china L.Zymolysis is the most critical factor on the hydrolytic yield of diosgenin.