细胞周期调节因子在乳腺导管增生性疾病及乳腺癌中的表达及意义

目的研究共济失调毛细血管扩张症突变蛋白(Ataxia-Telangiectasa mutated protein,ATM protein)、检测点激酶2(Checkpointskinase1,Chk2)及P53在乳腺浸润性导管癌(invasive ductal carcinoma,IDC)、导管内癌(ductal carcinoma in situ,DCIS)、非典型导管增生(atypical ductal hyperplasia,ADH)、癌旁正常乳腺组织(normal brease tissues adjacent to cancer,NBTAC)中的表达情况,探讨其在乳腺癌发生发展中的作用。方法采用免疫组化S-P法检测63例乳腺浸润性导管癌、39例导管内癌、42例非典型导管增生、56例癌旁正常乳腺组织中ATM、Chk2及P53的表达情况。结果 ATM、Chk2在乳腺浸润性导管癌中的表达明显低于癌旁正常乳腺组织;P53在乳腺浸润性导管癌中的表达明显高于癌旁正常乳腺组织;ATM、Chk2与P53表达呈显著负相关。结论乳腺浸润性癌ATM、Chk2表达缺失及P53的表达增高可能是乳腺癌发生、发展的重要因素之一,联合检测对早期乳腺癌的诊断及判断乳腺癌预后有指导意义。

Expression and Significance of Cell Cycle Regulators in Breast Intraductal Proliferative Diseases and Breast Carcinoma

Objective This study investigate the expression of cell cycle regulators, including ATM, Chk2 and p53 in breast carcinoma. Methods The expressions of ATM, Chk2 and p53 in 63 cases of breast invasive ductal carcinoma （BIDC）, 39 cases of breast carcinoma in situ （BIDC）, 42 cases of atypical ductal hyperplasia （ADH） and 56 cases of normal breast tissues adjacent to cancer （NBTAC） were observes by SP immunohistochemistry. Results The positive expression levels of ATM and Chk2 in BIDC were significantly lower than NBTAC （P〈0.05）. The positive expression level of P53 in BIDC was significantly higher than NBTAC （P〈0.05）. Conclusion These results suggest that down-regulation of ATM and Chk2, and over-expression of P53 are significantly related to genesis and progression of breast carcinoma. Combined determination of these three proteins may play an important role in diagnosis and prognosis of breast carcinoma.