高温应激致麒麟鸡(卷羽鸡)肝损伤试验动物模型的建立

本试验旨在建立急性应激致禽类肝损伤的试验动物模型.对麒麟鸡(卷羽鸡)进行持续高温应激后,检测血清中的肝损伤酶谷丙转氨酶(ALT)、天冬氨酸转氨酶(AST)、胆碱脂酶(CHE)和乳酸脱氢酶(LDH)活性的改变,观察肝脏病理组织学变化.结果显示,高温应激前期,麒麟鸡血清肝损伤酶AST和ALT活性极显著升高(P <0.01),高温应激后期,AST活性显著低于正常水平(P <0.05),而ALT活性极显著低于正常水平(P <0.01);持续高温应激期间,CHE的活性一直低于正常水平,高温应激后期,CHE活性极显著低于正常水平(P <0.01);在高温应激下LDH活性呈先下降后上升再下降的变化趋势,高温应激后期,LDH活性极显著高于正常水平(P <0.01).高温应激引起麒麟鸡肝细胞出现水泡变性、肝细胞核浓缩现象.结果表明,采用急性热应激可诱导麒麟鸡肝损伤试验模型,试验模型具备肝脏水泡变性和核浓缩病理特征.

Establishment of Experimental Animal Models of Kirin Chicken (Chicken Feather Volume) Liver Injury Induced by Heat Stress

The purpose of this study was to establish an experimental animal model of poultry liver injury induced by acute heat stress.We examined the changes of liver damage enzyme (alanine transaminase (ALT),aspartate transaminase (AST),cholinesterase (CHE),lactate dehydrogenase (LDH)) in Kirin chicken serum and observed histopathological changes in liver after continuous heat stress.The results showed that pre-heat stress caused an extremely significant increasing in the activity of Kirin chicken liver damage enzymes AST and ALT (P <0.01),heat stress late,the activity of AST was significantly lower than normal levels (P <0.05),while the activity of ALT was extremely significantly lower than normal levels (P <0.01);During the continuous heat stress,the activity of CHE had been lower than normal levels,heat stress late,the activity of CHE was extremely significantly lower than normal levels (P <0.01);The activity of LDH increased and then decreased after the first fall change after continuous heat stress,heat stress late,the activity of LDH was extremely significantly higher than normal levels (P <0.01).The histopathological lesions in liver were characterized by typical vesicular degeneration and hepatocyte nuclear enrichment in Kirin chicken after continuous heat stress.A Kirin chicken model of liver injury was successfully established by continuous heat stress,this experimental model of liver cells had vesicular degeneration and hepatocyte nuclear enrichment.