京海黄鸡杂交组合亲本鸡球虫抗性指标的比较

为研究京海黄鸡杂交组合亲本BB鸡与FF鸡鸡球虫病抗性的差异,试验选取BB和FF公鸡各30只,饲养至9日龄时,每个群体随机分为免疫组(n=15)和非免疫对照组(n=15),分组当天免疫组京海黄鸡杂交组合亲本按正典鸡球虫病四价活疫苗说明书要求接种鸡球虫疫苗,疫苗免疫后第8天采用生物素双抗体酶联免疫吸附法(ELISA)测定免疫组和非免疫对照组8个血浆球虫抗性指标,并进行差异显著性检验.结果显示,BB鸡免疫组过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和白介素17(IL-17)的含量显著高于FF鸡免疫组(P <0.05);BB杂交组合亲本免疫组CAT含量极显著高于对照组(P <0.01),SOD含量显著高于对照组(P <0.05);FF杂交组合亲本免疫组CAT和谷胱甘肽过氧化物酶(GSH-Px)含量均显著高于对照组(P <0.05).综合分析结果表明,BB鸡免疫效果优于FF鸡群体,BB鸡球虫病抗性优于FF鸡群体.

Comparison of Resistant Indexes of Coccidiosis between the Two Hybrid Parents of Jinghai Yellow Chicken

In order to investigate the differences of coccidiosis resistance between Jinghai Yellow chicken hybrid parental BB and FF chickens,30 experimental cocks of BB and FF were randomly divided into vaccine group (n=15) and control group (n=15) at 9-day-old,and then the plasmas of each group were separated from each chicken at 8 days post vaccination,and the resistant indexes were measured using biotin double antibody enzyme-linked immunosorbent assay (ELISA),the significant differences between groups were tested.The results showed that the CAT,SOD,IL-17 levels in BB vaccine group were significantly higher than that in FF vaccine group (P <0.05);The CAT level in BB vaccine group was very significantly higher than that in control group (P <0.01);The SOD level in BB vaccine group was significantly higher than that in control group (P <0.05);The CAT and GSH-Px levels in FF vaccine group were significantly higher than that in control group (P <0.05).Comprehensive analysis results shows that immune effects of BB vaccine group was superior to that of FF vaccine group,and coccidiosis resistance of BB chickens was superior to that of FF chickens.